Cell-mediated immune response in swine infected with Mycobacterium avium subsp. avium

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F11%3A%230000744" target="_blank" >RIV/00027162:_____/11:#0000744 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Cell-mediated immune response in swine infected with Mycobacterium avium subsp. avium

Popis výsledku v původním jazyce

We analysed porcine antigen-specific immunity six months after experimental infection with Mycobacterium avium subsp. avium (MAA) by measuring IFN-gamma production (ELISA, qRT-PCR and flow cytometry) and lymphocyte transformation test after in vitro re-stimulation with the MAA-derived antigen. In MAA-infected animals, IFN-gamma was increased at both mRNA and protein levels in response to the specific MAA antigen in the blood, spleen and mesenteric lymph nodes. Similarly, a positive antigen-driven response was detected by proliferation assay. Using flow cytometry, we found double-positive CD4+CD8+ lymphocytes as the major T lymphocyte subset producing IFN-gamma after specific re-stimulation. In conclusion, antigen-specific immune response in swine is retained for at least six months after infection with MAA. Furthermore, we identified the presence of antigen-specific double-positive CD4+CD8+ cells in MAA-infected pigs.

Název v anglickém jazyce

Cell-mediated immune response in swine infected with Mycobacterium avium subsp. avium

Popis výsledku anglicky

We analysed porcine antigen-specific immunity six months after experimental infection with Mycobacterium avium subsp. avium (MAA) by measuring IFN-gamma production (ELISA, qRT-PCR and flow cytometry) and lymphocyte transformation test after in vitro re-stimulation with the MAA-derived antigen. In MAA-infected animals, IFN-gamma was increased at both mRNA and protein levels in response to the specific MAA antigen in the blood, spleen and mesenteric lymph nodes. Similarly, a positive antigen-driven response was detected by proliferation assay. Using flow cytometry, we found double-positive CD4+CD8+ lymphocytes as the major T lymphocyte subset producing IFN-gamma after specific re-stimulation. In conclusion, antigen-specific immune response in swine is retained for at least six months after infection with MAA. Furthermore, we identified the presence of antigen-specific double-positive CD4+CD8+ cells in MAA-infected pigs.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GJ - Choroby a škůdci zvířat, veterinární medicina

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Veterinary Immunology and Immunopathology

ISSN

0165-2427

e-ISSN

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Svazek periodika

142

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

6

Strana od-do

107-112

Kód UT WoS článku

000292486400013

EID výsledku v databázi Scopus

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