Molecular characterization of a porcine sapovirus strain isolated from a piglet with diarrhoea: a case report

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F11%3A%230000770" target="_blank" >RIV/00027162:_____/11:#0000770 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Molecular characterization of a porcine sapovirus strain isolated from a piglet with diarrhoea: a case report

Popis výsledku v původním jazyce

Porcine sapoviruse have been considered as an aethiological agent of gastroenteritis in pigs. In this study, we therefore analysed 251 faecal samples obtained from diarrhoeic pigs in the Czech Republic during January 2005-June 2010 and tested them by negative staining electron microscopy for the presence of sapoviruses. Only one sample showed the presence of viral particles with characteristic sapovirus morphology. Presence of sapovirus was confirmed by RT-PCR assay with primers specific to sapoviral RNA polymerase gene and capsid gene. The phylogenetic analysis based on partial RNA polymerase sequence placed the new Czech isolate into the GVII genogroup of porcine sapoviruses, however, analysis of partial capsid gene sequence classified isolate as GIII of the genus Sapovirus. The findings of phylogenetic analysis indicate, that recombinant porcine sapovirus was identified. According to our knowledge this is the first description of porcine sapovirus in domestic pigs in the Czech Repub

Název v anglickém jazyce

Molecular characterization of a porcine sapovirus strain isolated from a piglet with diarrhoea: a case report

Popis výsledku anglicky

Porcine sapoviruse have been considered as an aethiological agent of gastroenteritis in pigs. In this study, we therefore analysed 251 faecal samples obtained from diarrhoeic pigs in the Czech Republic during January 2005-June 2010 and tested them by negative staining electron microscopy for the presence of sapoviruses. Only one sample showed the presence of viral particles with characteristic sapovirus morphology. Presence of sapovirus was confirmed by RT-PCR assay with primers specific to sapoviral RNA polymerase gene and capsid gene. The phylogenetic analysis based on partial RNA polymerase sequence placed the new Czech isolate into the GVII genogroup of porcine sapoviruses, however, analysis of partial capsid gene sequence classified isolate as GIII of the genus Sapovirus. The findings of phylogenetic analysis indicate, that recombinant porcine sapovirus was identified. According to our knowledge this is the first description of porcine sapovirus in domestic pigs in the Czech Repub

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GJ - Choroby a škůdci zvířat, veterinární medicina

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Veterinární Medicína

ISSN

0375-8427

e-ISSN

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Svazek periodika

56

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

7

Strana od-do

409-415

Kód UT WoS článku

000295252600007

EID výsledku v databázi Scopus

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