Association of attenuated mutants of Salmonella enterica serovar Enteritidis with porcine peripheral blood leukocytes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F11%3A%230000802" target="_blank" >RIV/00027162:_____/11:#0000802 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Association of attenuated mutants of Salmonella enterica serovar Enteritidis with porcine peripheral blood leukocytes

Popis výsledku v původním jazyce

We were interested in the association of attenuated mutants of Salmonella enterica serovar Enteritidis (with inactivated aroA, phoP, rfaL, rfaG, rfaC and fliC genes and delta SPI1-5 mutant) with subpopulations of porcine white blood cells (WBC). Using flow cytometry, we did not observe any difference in the interactions of the wild-type S. Enteritidis, aroA and phoP mutants with WBC. Delta SPI1-5 and fliC mutants had a minor defect in their association with granulocytes and monocytes. All three rfa mutants associated with granulocytes, monocytes and B-lymphocytes more than the wildtype S. Enteritidis did. Electron microscopy showed that the Salmonella-containing vacuole in the WBC infected with the rfa mutants, unlike all other strains, did not developinto a spacious phagosome. Intact lipopolysaccharide, but not the type III secretion system encoded by SPI-1, SPI-2 or the flagellar operon, is important for the initial interaction of S. Enteritidis with porcine leukocytes.

Název v anglickém jazyce

Association of attenuated mutants of Salmonella enterica serovar Enteritidis with porcine peripheral blood leukocytes

Popis výsledku anglicky

We were interested in the association of attenuated mutants of Salmonella enterica serovar Enteritidis (with inactivated aroA, phoP, rfaL, rfaG, rfaC and fliC genes and delta SPI1-5 mutant) with subpopulations of porcine white blood cells (WBC). Using flow cytometry, we did not observe any difference in the interactions of the wild-type S. Enteritidis, aroA and phoP mutants with WBC. Delta SPI1-5 and fliC mutants had a minor defect in their association with granulocytes and monocytes. All three rfa mutants associated with granulocytes, monocytes and B-lymphocytes more than the wildtype S. Enteritidis did. Electron microscopy showed that the Salmonella-containing vacuole in the WBC infected with the rfa mutants, unlike all other strains, did not developinto a spacious phagosome. Intact lipopolysaccharide, but not the type III secretion system encoded by SPI-1, SPI-2 or the flagellar operon, is important for the initial interaction of S. Enteritidis with porcine leukocytes.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GJ - Choroby a škůdci zvířat, veterinární medicina

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

FEMS MICROBIOLOGY LETTERS

ISSN

0378-1097

e-ISSN

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Svazek periodika

321

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

6

Strana od-do

37-42

Kód UT WoS článku

000292337200006

EID výsledku v databázi Scopus

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