Usability of a gamma interferon release assay in the diagnosis of naturally infected pigs with Mycobacterium avium subspecies hominissuis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F12%3A%230000920" target="_blank" >RIV/00027162:_____/12:#0000920 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1177/1040638711435141" target="_blank" >http://dx.doi.org/10.1177/1040638711435141</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1177/1040638711435141" target="_blank" >10.1177/1040638711435141</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Usability of a gamma interferon release assay in the diagnosis of naturally infected pigs with Mycobacterium avium subspecies hominissuis

Popis výsledku v původním jazyce

In the current study, the results of an intradermal tuberculin test and a gamma interferon (INF-?) release assay were compared based on the detection of interferon-gamma production after in vitro stimulation with Mycobacterium avium subsp. avium-specificantigen for the discrimination of pigs naturally infected with M. avium subsp. hominissuis. Fifty-five clinically healthy pigs were used in the study. Three of these were proven by culture and real-time quantitative polymerase chain reaction methods tobe infected with M. avium subsp. hominissuis (2 animals) and Mycobacterium xenopi (1 animal). No animals were positive by the tuberculin test. Both M. avium subsp. hominissuis-positive pigs were evaluated as positive by the INF-? release assay. Bacteriologically negative and M. xenopi-positive pigs were unresponsive in the INF-? release assay, indicating the specificity of the method. The results suggest that the INF-? release assay has a higher sensitivity than the tuberculin test and t

Název v anglickém jazyce

Usability of a gamma interferon release assay in the diagnosis of naturally infected pigs with Mycobacterium avium subspecies hominissuis

Popis výsledku anglicky

In the current study, the results of an intradermal tuberculin test and a gamma interferon (INF-?) release assay were compared based on the detection of interferon-gamma production after in vitro stimulation with Mycobacterium avium subsp. avium-specificantigen for the discrimination of pigs naturally infected with M. avium subsp. hominissuis. Fifty-five clinically healthy pigs were used in the study. Three of these were proven by culture and real-time quantitative polymerase chain reaction methods tobe infected with M. avium subsp. hominissuis (2 animals) and Mycobacterium xenopi (1 animal). No animals were positive by the tuberculin test. Both M. avium subsp. hominissuis-positive pigs were evaluated as positive by the INF-? release assay. Bacteriologically negative and M. xenopi-positive pigs were unresponsive in the INF-? release assay, indicating the specificity of the method. The results suggest that the INF-? release assay has a higher sensitivity than the tuberculin test and t

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GJ - Choroby a škůdci zvířat, veterinární medicina

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Veterinary Diagnostic Investigation

ISSN

1040-6387

e-ISSN

—

Svazek periodika

24

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

3

Strana od-do

376-378

Kód UT WoS článku

000307114500021

EID výsledku v databázi Scopus

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