Experimental infection of gnotobiotic piglets with Escherichia coli strains positive for EAST1 and AIDA

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F13%3A%230000964" target="_blank" >RIV/00027162:_____/13:#0000964 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.vetimm.2012.09.011" target="_blank" >http://dx.doi.org/10.1016/j.vetimm.2012.09.011</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.vetimm.2012.09.011" target="_blank" >10.1016/j.vetimm.2012.09.011</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Experimental infection of gnotobiotic piglets with Escherichia coli strains positive for EAST1 and AIDA

Popis výsledku v původním jazyce

The virulence factors EAST1 and AIDA are often detected in ETEC/VTEC strains isolated from pigs and their role in diarrhoeal infections is discussed. In order to elucidate the pathogenesis of AIDA, the colonisation patterns of F4 positive and AIDA positive strains were investigated. Two wild-type Escherichia coli strains AIDA/EAST1 and F4/EAST1 isolated from diarrhoeal piglets were used for animal experiment to evaluate the ability of the EAST1 toxin to be involved in induction of diarrhoea. Gnotobioticpiglets were supplemented with normal porcine serum and orally inoculated with the strains. Faecal bacterial shedding of the challenge strains was observed during the experiment. Light microscopy and scanning electron microscopy were used to detect thecolonisation pattern of both challenge strains. Although bacterial isolation demonstrated shedding of the challenge strains until the end of the experiment, diarrhoea did not develop in any piglet. Based on histological examination, pigle

Název v anglickém jazyce

Experimental infection of gnotobiotic piglets with Escherichia coli strains positive for EAST1 and AIDA

Popis výsledku anglicky

The virulence factors EAST1 and AIDA are often detected in ETEC/VTEC strains isolated from pigs and their role in diarrhoeal infections is discussed. In order to elucidate the pathogenesis of AIDA, the colonisation patterns of F4 positive and AIDA positive strains were investigated. Two wild-type Escherichia coli strains AIDA/EAST1 and F4/EAST1 isolated from diarrhoeal piglets were used for animal experiment to evaluate the ability of the EAST1 toxin to be involved in induction of diarrhoea. Gnotobioticpiglets were supplemented with normal porcine serum and orally inoculated with the strains. Faecal bacterial shedding of the challenge strains was observed during the experiment. Light microscopy and scanning electron microscopy were used to detect thecolonisation pattern of both challenge strains. Although bacterial isolation demonstrated shedding of the challenge strains until the end of the experiment, diarrhoea did not develop in any piglet. Based on histological examination, pigle

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Veterinary Immunology and Immunopathology

ISSN

0165-2427

e-ISSN

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Svazek periodika

152

Číslo periodika v rámci svazku

1-2

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

6

Strana od-do

176-182

Kód UT WoS článku

000316535000024

EID výsledku v databázi Scopus

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