The Role of the qacA Gene in Mediating Resistance to Quaternary Ammonium Compounds

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F13%3A%230000970" target="_blank" >RIV/00027162:_____/13:#0000970 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1089/mdr.2012.0154" target="_blank" >http://dx.doi.org/10.1089/mdr.2012.0154</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1089/mdr.2012.0154" target="_blank" >10.1089/mdr.2012.0154</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The Role of the qacA Gene in Mediating Resistance to Quaternary Ammonium Compounds

Popis výsledku v původním jazyce

Conditions facilitating resistance to quaternary ammonium compounds (QACs) were investigated in Staphylococcus aureus SK982 exposed to benzalkonium chloride (BAC; a member of QACs) under various circumstances. S. aureus SK982 carrying the qacA gene encoding for resistance to QACs was grown in the presence of stable or gradually increasing concentrations of BAC, or it was exposed to this antiseptic in the exponential phase of growth. Bacteria cultivated in the highest BAC concentrations that did not retard their growth comparing to the untreated control were subjected to real-time quantitative polymerase chain reaction analysis for relative expression of the efflux genes qacA and norA. Under such conditions, S. aureus SK982 tolerated a relatively low stable concentration of BAC (1.22 mg/L) when compared with a gradually increasing antiseptic concentration (tolerance of 4.88mg/L). However, in both cases, qacA expression was not significant. The culture exposed in the exponential phase of

Název v anglickém jazyce

The Role of the qacA Gene in Mediating Resistance to Quaternary Ammonium Compounds

Popis výsledku anglicky

Conditions facilitating resistance to quaternary ammonium compounds (QACs) were investigated in Staphylococcus aureus SK982 exposed to benzalkonium chloride (BAC; a member of QACs) under various circumstances. S. aureus SK982 carrying the qacA gene encoding for resistance to QACs was grown in the presence of stable or gradually increasing concentrations of BAC, or it was exposed to this antiseptic in the exponential phase of growth. Bacteria cultivated in the highest BAC concentrations that did not retard their growth comparing to the untreated control were subjected to real-time quantitative polymerase chain reaction analysis for relative expression of the efflux genes qacA and norA. Under such conditions, S. aureus SK982 tolerated a relatively low stable concentration of BAC (1.22 mg/L) when compared with a gradually increasing antiseptic concentration (tolerance of 4.88mg/L). However, in both cases, qacA expression was not significant. The culture exposed in the exponential phase of

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

MICROBIAL DRUG RESISTANCE

ISSN

1076-6294

e-ISSN

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Svazek periodika

19

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

4

Strana od-do

160-167

Kód UT WoS článku

000319399900002

EID výsledku v databázi Scopus

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