Expression of integrin beta 2 (ITGB2) and zona pellucida glycoproteins (ZP3, ZP3 alpha) in developmentally competent and incompetent porcine oocytes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F14%3A%230001190" target="_blank" >RIV/00027162:_____/14:#0001190 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Expression of integrin beta 2 (ITGB2) and zona pellucida glycoproteins (ZP3, ZP3 alpha) in developmentally competent and incompetent porcine oocytes

Popis výsledku v původním jazyce

The fertilization potential of mammalian oocytes may be regulated at the molecular level by the expression of species-specific sperm-egg interaction molecules, whose activities and/or cellular distribution determine the recognition and fusion of gemetes.Although there exist studies on the expression of integrins (ITGs) and zona pellucida glycoproteins (ZPs) in developmentally fully competent oocytes, the mRNA levels encoding these proteins in immature and developmentally incompetent porcine oocytes have to be elucidated. Therefore, our aim was to determine the expression of ITGB2, ZP3, and ZP3 alpha mRNAs in porcine oocytes before in vitro maturation (IVM), in oocytes stained with BCB test but colorless, and in BCB positive oocytes after IVM. Porcinecumulus-oocyte complexes (COCs) were collected from 32 crossbred Landrace gilts, and then separated into three groups: (i) oocytes analyzed immediately after collection (n = 50), (ii) oocytes stained with brilliant cresyl blue (BCB+) and

Název v anglickém jazyce

Expression of integrin beta 2 (ITGB2) and zona pellucida glycoproteins (ZP3, ZP3 alpha) in developmentally competent and incompetent porcine oocytes

Popis výsledku anglicky

The fertilization potential of mammalian oocytes may be regulated at the molecular level by the expression of species-specific sperm-egg interaction molecules, whose activities and/or cellular distribution determine the recognition and fusion of gemetes.Although there exist studies on the expression of integrins (ITGs) and zona pellucida glycoproteins (ZPs) in developmentally fully competent oocytes, the mRNA levels encoding these proteins in immature and developmentally incompetent porcine oocytes have to be elucidated. Therefore, our aim was to determine the expression of ITGB2, ZP3, and ZP3 alpha mRNAs in porcine oocytes before in vitro maturation (IVM), in oocytes stained with BCB test but colorless, and in BCB positive oocytes after IVM. Porcinecumulus-oocyte complexes (COCs) were collected from 32 crossbred Landrace gilts, and then separated into three groups: (i) oocytes analyzed immediately after collection (n = 50), (ii) oocytes stained with brilliant cresyl blue (BCB+) and

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GI - Šlechtění a plemenářství hospodářských zvířat

OECD FORD obor

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Projekt

<a href="/cs/project/7AMB13PL052" target="_blank" >7AMB13PL052: Cytoplazmatické zrání prasečích oocytů během folikulogeneze</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

MEDYCYNA WETERYNARYJNA-VETERINARY MEDICINE-SCIENCE AND PRACTICE

ISSN

0025-8628

e-ISSN

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Svazek periodika

70

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

PL - Polská republika

Počet stran výsledku

5

Strana od-do

417-421

Kód UT WoS článku

000338272600008

EID výsledku v databázi Scopus

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