African swine fever virus infects macrophages, the natural host cells, via clathrin- and cholesterol-dependent endocytosis

Kód výsledku v IS VaVaI

RIV/00027162:_____/15:#0001278 - isvavai.cz

Výsledek na webu

http://www.journals.elsevier.com/virus-research

DOI - Digital Object Identifier

10.1016/j.virusres.2015.01.022

Jazyk výsledku

angličtina

Název v původním jazyce

African swine fever virus infects macrophages, the natural host cells, via clathrin- and cholesterol-dependent endocytosis

Popis výsledku v původním jazyce

The main cellular target for African swine fever virus (ASFV) is the porcine macrophage. However, existing data about the early phases of infection were previously characterized in non-leukocyte cells such as Vero cells. Here, we report that ASFV entersthe natural host cell using dynamin-dependent and clathrin-mediated endocytosis. This pathway is strongly pH-dependent during the first steps of infection in porcine macrophages. We investigated the effect of drugs inhibiting several endocytic pathways in macrophages and compared ASFV with vaccinia virus (VV), which apparently involves different entry pathways. The presence of cholesterol in cellular membranes was found to be essential for a productive ASFV infection while actin-dependent endocytosis and the participation of phosphoinositide-3-kinase (PI3K) activity were other cellular factors required in the process of viral entry. These findings improved our understanding of the ASFV interactions with macrophages that allow for succes

Název v anglickém jazyce

African swine fever virus infects macrophages, the natural host cells, via clathrin- and cholesterol-dependent endocytosis

Popis výsledku anglicky

The main cellular target for African swine fever virus (ASFV) is the porcine macrophage. However, existing data about the early phases of infection were previously characterized in non-leukocyte cells such as Vero cells. Here, we report that ASFV entersthe natural host cell using dynamin-dependent and clathrin-mediated endocytosis. This pathway is strongly pH-dependent during the first steps of infection in porcine macrophages. We investigated the effect of drugs inhibiting several endocytic pathways in macrophages and compared ASFV with vaccinia virus (VV), which apparently involves different entry pathways. The presence of cholesterol in cellular membranes was found to be essential for a productive ASFV infection while actin-dependent endocytosis and the participation of phosphoinositide-3-kinase (PI3K) activity were other cellular factors required in the process of viral entry. These findings improved our understanding of the ASFV interactions with macrophages that allow for succes

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GJ - Choroby a škůdci zvířat, veterinární medicina

OECD FORD obor

—

Projekt

LO1218: Zdravé zvíře jako zdroj zdravé potraviny

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Virus research

ISSN

0168-1702

e-ISSN

—

Svazek periodika

200

Číslo periodika v rámci svazku

Mar 16

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

11

Strana od-do

45-55

Kód UT WoS článku

000351798800007

EID výsledku v databázi Scopus

—