Molecular diagnosis of bovine tuberculosis in bovine and human samples: implications for zoonosis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F15%3A%230001303" target="_blank" >RIV/00027162:_____/15:#0001303 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.futuremedicine.com/loi/fmb" target="_blank" >http://www.futuremedicine.com/loi/fmb</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2217/FMB.14.139" target="_blank" >10.2217/FMB.14.139</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Molecular diagnosis of bovine tuberculosis in bovine and human samples: implications for zoonosis

Popis výsledku v původním jazyce

Aim: To develop emerging diagnostic technique for bovine tuberculosis and to identify its potential risk factors. Materials & methods: Bacterial genomic DNA was isolated from bovine milk and human sputum samples and subjected to PCR using specific primer pairs. PCR results were validated using bacteriological cultures. Results: PCR amplification of the targeted DNA fragment of Mycobacterium bovis was successful in 12.33% (37/300) of the bovine samples. Interestingly, 500-bp DNA fragment was also amplified in 6.67% (6/90) of the sputum indicating the possibility of zoonotic transmission. Rearing of livestock in household, unpasteurized milk consumption and smoking were identified as potential risk factors. Conclusion: Results of the study may add value to bovine tuberculosis eradication campaigns to achieve the One Health initiative.

Název v anglickém jazyce

Molecular diagnosis of bovine tuberculosis in bovine and human samples: implications for zoonosis

Popis výsledku anglicky

Aim: To develop emerging diagnostic technique for bovine tuberculosis and to identify its potential risk factors. Materials & methods: Bacterial genomic DNA was isolated from bovine milk and human sputum samples and subjected to PCR using specific primer pairs. PCR results were validated using bacteriological cultures. Results: PCR amplification of the targeted DNA fragment of Mycobacterium bovis was successful in 12.33% (37/300) of the bovine samples. Interestingly, 500-bp DNA fragment was also amplified in 6.67% (6/90) of the sputum indicating the possibility of zoonotic transmission. Rearing of livestock in household, unpasteurized milk consumption and smoking were identified as potential risk factors. Conclusion: Results of the study may add value to bovine tuberculosis eradication campaigns to achieve the One Health initiative.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GJ - Choroby a škůdci zvířat, veterinární medicina

OECD FORD obor

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Projekt

—

Návaznosti

N - Vyzkumna aktivita podporovana z neverejnych zdroju

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Future Microbiology

ISSN

1746-0913

e-ISSN

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Svazek periodika

10

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

9

Strana od-do

527-535

Kód UT WoS článku

000352805200009

EID výsledku v databázi Scopus

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