Detection of genes associated with developmental competence of bovine oocytes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F16%3AN0000038" target="_blank" >RIV/00027162:_____/16:N0000038 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985904:_____/16:00460683

Výsledek na webu

<a href="https://www.researchgate.net/publication/289584715_Detection_of_genes_associated_with_developmental_competence_of_bovine_oocytes" target="_blank" >https://www.researchgate.net/publication/289584715_Detection_of_genes_associated_with_developmental_competence_of_bovine_oocytes</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.anireprosci.2016.01.004" target="_blank" >10.1016/j.anireprosci.2016.01.004</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Detection of genes associated with developmental competence of bovine oocytes

Popis výsledku v původním jazyce

The developmental competence of oocytes is acquired progressively during folliculogenesis and is linked to follicular size. It has been documented that oocytes originating from larger follicles exhibit a greater ability to develop to the blastocyst stage. The differences in cytoplasmic factors such as mRNA transcripts could explain the differences in oocyte developmental potential. We used bovine oligonucleotide microarrays to characterize differences between the gene expression profiles of germinal vesicle stage (GV) oocytes with greater developmental competence from medium follicles (MF) and those with less developmental competence from small follicles (SF). After normalizing the microarray data, our analysis found differences in the level of 60 transcripts (>= 1.4 fold), corresponding to 49 upregulated and 11 downregulated transcripts in MF oocytes compared to SF oocytes. The gene expression data were classified according to gene ontology, the majority of the genes were associated with the regulation of transcription, translation, the cell cycle, and mitochondria) activity. A subset of 16 selected genes was validated for GV oocytes by quantitative real-time RT-PCR; significant differences (P<0.01) were found in the level of TAF1A,MTRF1L, ATP5C1, UBL5 and MAP3K13 between the MF and SF oocytes. After maturation the transcript level remained stable for ATP5F1, BRD7, and UBL5 in both oocyte categories. The transcript level of another 13 genes substantially dropped in the MF and/or SF oocytes. It can be concluded that the developmental competence of bovine oocytes and embryos may be a quantitative trait dependent on small changes in the transcription profiles of many genes).

Název v anglickém jazyce

Detection of genes associated with developmental competence of bovine oocytes

Popis výsledku anglicky

The developmental competence of oocytes is acquired progressively during folliculogenesis and is linked to follicular size. It has been documented that oocytes originating from larger follicles exhibit a greater ability to develop to the blastocyst stage. The differences in cytoplasmic factors such as mRNA transcripts could explain the differences in oocyte developmental potential. We used bovine oligonucleotide microarrays to characterize differences between the gene expression profiles of germinal vesicle stage (GV) oocytes with greater developmental competence from medium follicles (MF) and those with less developmental competence from small follicles (SF). After normalizing the microarray data, our analysis found differences in the level of 60 transcripts (>= 1.4 fold), corresponding to 49 upregulated and 11 downregulated transcripts in MF oocytes compared to SF oocytes. The gene expression data were classified according to gene ontology, the majority of the genes were associated with the regulation of transcription, translation, the cell cycle, and mitochondria) activity. A subset of 16 selected genes was validated for GV oocytes by quantitative real-time RT-PCR; significant differences (P<0.01) were found in the level of TAF1A,MTRF1L, ATP5C1, UBL5 and MAP3K13 between the MF and SF oocytes. After maturation the transcript level remained stable for ATP5F1, BRD7, and UBL5 in both oocyte categories. The transcript level of another 13 genes substantially dropped in the MF and/or SF oocytes. It can be concluded that the developmental competence of bovine oocytes and embryos may be a quantitative trait dependent on small changes in the transcription profiles of many genes).

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GI - Šlechtění a plemenářství hospodářských zvířat

OECD FORD obor

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Projekt

<a href="/cs/project/LD14104" target="_blank" >LD14104: Vliv stimulace mitochondriální aktivity na vývojový potenciál savčích oocytů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Animal Reproduction Science

ISSN

0378-4320

e-ISSN

—

Svazek periodika

Neuveden

Číslo periodika v rámci svazku

166

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

14

Strana od-do

58-71

Kód UT WoS článku

000371189400008

EID výsledku v databázi Scopus

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