Prevalence and characteristics of Salmonella species isolated from captive reptiles in the Czech Republic

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F17%3AN0000025" target="_blank" >RIV/00027162:_____/17:N0000025 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62157124:16170/17:43875880

Výsledek na webu

<a href="http://www.agriculturejournals.cz/publicFiles/223420.pdf" target="_blank" >http://www.agriculturejournals.cz/publicFiles/223420.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17221/44/2017-VETMED" target="_blank" >10.17221/44/2017-VETMED</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Prevalence and characteristics of Salmonella species isolated from captive reptiles in the Czech Republic

Popis výsledku v původním jazyce

This study was aimed at determining the prevalence and characterising the strains of Salmonella species in various captive reptiles in the Czech Republic. A total of 211 samples of cloacal swabs from lizards, chelonians and snakes, and 14 swabs from terraria surfaces were collected between November 2014 and July 2015. After isolation according to the reference method (EN ISO), Salmonella spp. isolates were characterised using serotyping and macrorestriction analysis followed by pulsed field gel electrophoresis and antimicrobial susceptibility testing. Altogether, 39 isolates were obtained from 29 (19%) reptiles and from terraria surfaces. Among the different reptilian species, Salmonella spp. were found in 22 (25.6%) lizards, three (17.6%) snakes and four (8%) chelonians with 31 isolates classified as Salmonella enterica subspecies enterica and eight isolates classified as Salmonella enterica subsp. salamae. In total, 14 different serotypes were detected, with the most frequent serotypes being Salmonella Oranienburg, S. Fluntern, S. Tennessee and S. Cotham. Resistance to one antimicrobial agent (ampicillin, tetracycline or streptomycin) was detected in five isolates. The results of the macrorestriction analysis within the serotype groups showed varying level of heterogeneity. This study confirms that reptiles kept as pets can be both carriers and reservoirs of Salmonella spp., and that they can harbour various serotypes with intermittent excretion of the bacteria in faeces. Half of the detected serotypes have been involved in human reptileassociated salmonellosis cases in the past. S. enterica subsp. salamae serotype O:1,13,23;H: z29;H:1,5, monophasic S. enterica subsp. salamae serotype O:40;H:g,t; H:-and its biphasic form (S. enterica subsp. salamae serotype O:40; H:g,t;H:1,5) have apparently been isolated from reptiles for the first time in this study.

Název v anglickém jazyce

Prevalence and characteristics of Salmonella species isolated from captive reptiles in the Czech Republic

Popis výsledku anglicky

This study was aimed at determining the prevalence and characterising the strains of Salmonella species in various captive reptiles in the Czech Republic. A total of 211 samples of cloacal swabs from lizards, chelonians and snakes, and 14 swabs from terraria surfaces were collected between November 2014 and July 2015. After isolation according to the reference method (EN ISO), Salmonella spp. isolates were characterised using serotyping and macrorestriction analysis followed by pulsed field gel electrophoresis and antimicrobial susceptibility testing. Altogether, 39 isolates were obtained from 29 (19%) reptiles and from terraria surfaces. Among the different reptilian species, Salmonella spp. were found in 22 (25.6%) lizards, three (17.6%) snakes and four (8%) chelonians with 31 isolates classified as Salmonella enterica subspecies enterica and eight isolates classified as Salmonella enterica subsp. salamae. In total, 14 different serotypes were detected, with the most frequent serotypes being Salmonella Oranienburg, S. Fluntern, S. Tennessee and S. Cotham. Resistance to one antimicrobial agent (ampicillin, tetracycline or streptomycin) was detected in five isolates. The results of the macrorestriction analysis within the serotype groups showed varying level of heterogeneity. This study confirms that reptiles kept as pets can be both carriers and reservoirs of Salmonella spp., and that they can harbour various serotypes with intermittent excretion of the bacteria in faeces. Half of the detected serotypes have been involved in human reptileassociated salmonellosis cases in the past. S. enterica subsp. salamae serotype O:1,13,23;H: z29;H:1,5, monophasic S. enterica subsp. salamae serotype O:40;H:g,t; H:-and its biphasic form (S. enterica subsp. salamae serotype O:40; H:g,t;H:1,5) have apparently been isolated from reptiles for the first time in this study.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40301 - Veterinary science

Projekt

<a href="/cs/project/LO1218" target="_blank" >LO1218: Zdravé zvíře jako zdroj zdravé potraviny</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Veterinární medicína

ISSN

0375-8427

e-ISSN

—

Svazek periodika

62

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

14

Strana od-do

456-469

Kód UT WoS článku

000408605000005

EID výsledku v databázi Scopus

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