Molecular Characterization of OXA-48-Like- Producing Enterobacteriaceae in the Czech Republic and Evidence for Horizontal Transfer of pOXA-48-Like Plasmids

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F17%3AN0000049" target="_blank" >RIV/00027162:_____/17:N0000049 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11140/17:10331747 RIV/00216208:11150/17:10331747 RIV/75010330:_____/17:00011790

Výsledek na webu

<a href="http://aac.asm.org/content/early/2016/11/08/AAC.01889-16.abstract" target="_blank" >http://aac.asm.org/content/early/2016/11/08/AAC.01889-16.abstract</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1128/AAC.01889-16" target="_blank" >10.1128/AAC.01889-16</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Molecular Characterization of OXA-48-Like- Producing Enterobacteriaceae in the Czech Republic and Evidence for Horizontal Transfer of pOXA-48-Like Plasmids

Popis výsledku v původním jazyce

The aim of this study was to characterize the first cases and outbreaks of OXA-48-like-producing Enterobacteriaceae recovered from hospital settings in the Czech Republic. From 2013 to 2015, 22 Klebsiella pneumoniae isolates, 3 Escherichia coli isolates, and 1 Enterobacter cloacae isolate producing OXA-48-like carbapenemases were isolated from 20 patients. Four of the patients were colonized or infected by two or three different OXA-48-like producers. The K. pneumoniae isolates were classified into nine sequence types (STs), with ST101 being predominant (n = 8). The E. coli isolates were of different STs, while the E. cloacae isolate belonged to ST109. Twenty-four isolates carried bla(OXA-48), while two isolates carried bla(OXA-181) or bla(OXA-232). Almost all isolates (n = 22) carried bla(OXA-48)-positive plasmids of a similar size (similar to 60 kb), except the two isolates producing OXA-181 or OXA-232. In an ST45 K. pneumoniae isolate and an ST38 E. coli isolate, S1 nuclease profiling plus hybridization indicated a chromosomal location of bla(OXA-48). Sequencing showed that the majority of bla(OXA-48)-carrying plasmids exhibited high degrees of identity with the pOXA-48-like plasmid pE71T. Additionally, two novel pE71T derivatives, pOXA48_ 30715 and pOXA-48_30891, were observed. The bla(OXA-181)-carrying plasmid was identical to the IncX3 plasmid pOXA181_EC14828, while the bla(OXA-232)-carrying plasmid was a ColE2-type plasmid, being a novel derivative of pOXA-232. Finally, sequencing data showed that the ST45 K. pneumoniae and ST38 E. coli isolates harbored the IS1R-based composite transposon Tn6237 containing bla(OXA-48) integrated into their chromosomes. These findings underlined that the horizontal transfer of pOXA-48-like plasmids has played a major role in the dissemination of bla(OXA-48) in the Czech Republic. In combination with the difficulties with their detection, OXA-48 producers constitute an important public threat.

Název v anglickém jazyce

Molecular Characterization of OXA-48-Like- Producing Enterobacteriaceae in the Czech Republic and Evidence for Horizontal Transfer of pOXA-48-Like Plasmids

Popis výsledku anglicky

The aim of this study was to characterize the first cases and outbreaks of OXA-48-like-producing Enterobacteriaceae recovered from hospital settings in the Czech Republic. From 2013 to 2015, 22 Klebsiella pneumoniae isolates, 3 Escherichia coli isolates, and 1 Enterobacter cloacae isolate producing OXA-48-like carbapenemases were isolated from 20 patients. Four of the patients were colonized or infected by two or three different OXA-48-like producers. The K. pneumoniae isolates were classified into nine sequence types (STs), with ST101 being predominant (n = 8). The E. coli isolates were of different STs, while the E. cloacae isolate belonged to ST109. Twenty-four isolates carried bla(OXA-48), while two isolates carried bla(OXA-181) or bla(OXA-232). Almost all isolates (n = 22) carried bla(OXA-48)-positive plasmids of a similar size (similar to 60 kb), except the two isolates producing OXA-181 or OXA-232. In an ST45 K. pneumoniae isolate and an ST38 E. coli isolate, S1 nuclease profiling plus hybridization indicated a chromosomal location of bla(OXA-48). Sequencing showed that the majority of bla(OXA-48)-carrying plasmids exhibited high degrees of identity with the pOXA-48-like plasmid pE71T. Additionally, two novel pE71T derivatives, pOXA48_ 30715 and pOXA-48_30891, were observed. The bla(OXA-181)-carrying plasmid was identical to the IncX3 plasmid pOXA181_EC14828, while the bla(OXA-232)-carrying plasmid was a ColE2-type plasmid, being a novel derivative of pOXA-232. Finally, sequencing data showed that the ST45 K. pneumoniae and ST38 E. coli isolates harbored the IS1R-based composite transposon Tn6237 containing bla(OXA-48) integrated into their chromosomes. These findings underlined that the horizontal transfer of pOXA-48-like plasmids has played a major role in the dissemination of bla(OXA-48) in the Czech Republic. In combination with the difficulties with their detection, OXA-48 producers constitute an important public threat.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30302 - Epidemiology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Antimicrobial Agents and Chemotherapy

ISSN

0066-4804

e-ISSN

—

Svazek periodika

61

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

e01889-16

Kód UT WoS článku

000394102500045

EID výsledku v databázi Scopus

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