Seed Germination Test as a Potential Pregnancy Diagnosis Method for Domestic Cattle

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F17%3AN0000089" target="_blank" >RIV/00027162:_____/17:N0000089 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60460709:41340/17:75567

Výsledek na webu

<a href="http://www.agrojournal.org/23/03-15.pdf" target="_blank" >http://www.agrojournal.org/23/03-15.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Seed Germination Test as a Potential Pregnancy Diagnosis Method for Domestic Cattle

Popis výsledku v původním jazyce

The timely diagnosis of pregnancy is of paramount importance in cattle management and non-invasive diagnostic methods may help to improve the welfare of animals. In the present study, we evaluated the seed germination pregnancy test combined with urinalysis. Urine samples were obtained from 20 non-pregnant Czech Fleckvieh heifers and from 20 artifi cially inseminated heifers during six phases of pregnancy: 0–50 days, 51–100 days, 101–150 days, 151–200 days, 201-250 days, and over 250 days. The seed germination test was conducted in all animals using mung beans (Vigna radiata) and wheat seeds (Triticum spelta). A total of 143 and 150 urine samples from pregnant and non-pregnant control heifers, respectively, were processed, and 37,218 germinated seeds were evaluated. The number of germinated mung beans and wheat seeds were counted over five consecutive days, and the shoot lengths were measured on day 5. On day 1 of the test, there was a higher number of germinated mung beans in urine samples obtained during the fi rst half of pregnancy. The urine of heifers in the later stages of pregnancy showed a reduced number of germinated mung beans (P < 0.01). A negative infl uence of pregnancy on shoot length was also only noticeable in mung beans (P < 0.0001). On the other hand, the shortest shoots were measured in wheat seeds kept in the urine of non-pregnant heifers. Urine pH was higher in pregnant heifers than in non-pregnant heifers (P < 0.0001) but germination of wheat seeds was not signifi cantly (P > 0.05) infl uenced by any urinalysis parameter, as measured using diagnostic urine strips. Thus the seed germination test evaluating the shoot length of mung beans can represent a cheap and non-invasive method to distinguish pregnant from non-pregnant heifers.

Název v anglickém jazyce

Seed Germination Test as a Potential Pregnancy Diagnosis Method for Domestic Cattle

Popis výsledku anglicky

The timely diagnosis of pregnancy is of paramount importance in cattle management and non-invasive diagnostic methods may help to improve the welfare of animals. In the present study, we evaluated the seed germination pregnancy test combined with urinalysis. Urine samples were obtained from 20 non-pregnant Czech Fleckvieh heifers and from 20 artifi cially inseminated heifers during six phases of pregnancy: 0–50 days, 51–100 days, 101–150 days, 151–200 days, 201-250 days, and over 250 days. The seed germination test was conducted in all animals using mung beans (Vigna radiata) and wheat seeds (Triticum spelta). A total of 143 and 150 urine samples from pregnant and non-pregnant control heifers, respectively, were processed, and 37,218 germinated seeds were evaluated. The number of germinated mung beans and wheat seeds were counted over five consecutive days, and the shoot lengths were measured on day 5. On day 1 of the test, there was a higher number of germinated mung beans in urine samples obtained during the fi rst half of pregnancy. The urine of heifers in the later stages of pregnancy showed a reduced number of germinated mung beans (P < 0.01). A negative infl uence of pregnancy on shoot length was also only noticeable in mung beans (P < 0.0001). On the other hand, the shortest shoots were measured in wheat seeds kept in the urine of non-pregnant heifers. Urine pH was higher in pregnant heifers than in non-pregnant heifers (P < 0.0001) but germination of wheat seeds was not signifi cantly (P > 0.05) infl uenced by any urinalysis parameter, as measured using diagnostic urine strips. Thus the seed germination test evaluating the shoot length of mung beans can represent a cheap and non-invasive method to distinguish pregnant from non-pregnant heifers.

Druh

J_SC - Článek v periodiku v databázi SCOPUS

CEP obor

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OECD FORD obor

40201 - Animal and dairy science; (Animal biotechnology to be 4.4)

Projekt

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Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Bulgarian Journal of Agriculture Science

ISSN

1310-0351

e-ISSN

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Svazek periodika

23

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

BG - Bulharská republika

Počet stran výsledku

9

Strana od-do

453–461

Kód UT WoS článku

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EID výsledku v databázi Scopus

2-s2.0-85021299373