The effect of IgY antibodies on Mycobacterium avium subsp. paratuberculosis infection in mice

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The effect of IgY antibodies on Mycobacterium avium subsp. paratuberculosis infection in mice

Popis výsledku v původním jazyce

Abstrakt in Abstract book, 6th European Veterinary Immunology Workshop, EVIW 2018, 5. – 7. 9. 2018, Utrecht, The Netherlands. Mycobacterium avium subspecies paratuberculosis (MAP) is a pathogen responsible for Johne’s disease in cattle and other ruminants. After entry into the host, MAP survives in macrophages of intestinal wall and leads to chronic gastrointestinal tract infection and production of pro-inflammatory cytokines. The aim of this study was to test whether an egg yolk-specific IgY antibodies can affect the course of an experimental MAP infection in mouse model. Mice were infected perorally with MAP (108/100μl, CAM 6381 strain) and 2 days in a row revaccinated. Half of the animals were then treated with IgY. After three and twenty-eight days, group of animals were euthanized and samples of spleen, liver and small intestine were taken for the molecular analysis of mRNA expression and for the supplementary haematological, histological and bacteriological analysis. The presence of pathogen and changes in the inflammatory response were detected by RT-qPCR. Inflammatory cytokine expression was determined, concerning specifically cytokines IL-1β, IL-17, IFNγ and TNFα. Based on the RT-qPCR method performed in samples of spleen, liver and small intestine we can state that MAP effectively infected only small intestine and the infection did not spread further. Moreover, IgY reduced the MAP levels in small intestine. After the decrease of MAP level in small intestine due to IgY addition, the IL-1β, IL-17, IFNγ and TNFα cytokine expression was measured. Unfortunately, the effect of IgY downregulating the expression of cytokines was not confirmed. Inflammatory cytokine modulation during infection by IgY was in some cases also detected in spleen and liver. However, MAP did not successfully infected this organs therefore it is probably the total response of mice to infection and the cause of this condition remains to be elucidated.

Název v anglickém jazyce

The effect of IgY antibodies on Mycobacterium avium subsp. paratuberculosis infection in mice

Popis výsledku anglicky

Abstrakt in Abstract book, 6th European Veterinary Immunology Workshop, EVIW 2018, 5. – 7. 9. 2018, Utrecht, The Netherlands. Mycobacterium avium subspecies paratuberculosis (MAP) is a pathogen responsible for Johne’s disease in cattle and other ruminants. After entry into the host, MAP survives in macrophages of intestinal wall and leads to chronic gastrointestinal tract infection and production of pro-inflammatory cytokines. The aim of this study was to test whether an egg yolk-specific IgY antibodies can affect the course of an experimental MAP infection in mouse model. Mice were infected perorally with MAP (108/100μl, CAM 6381 strain) and 2 days in a row revaccinated. Half of the animals were then treated with IgY. After three and twenty-eight days, group of animals were euthanized and samples of spleen, liver and small intestine were taken for the molecular analysis of mRNA expression and for the supplementary haematological, histological and bacteriological analysis. The presence of pathogen and changes in the inflammatory response were detected by RT-qPCR. Inflammatory cytokine expression was determined, concerning specifically cytokines IL-1β, IL-17, IFNγ and TNFα. Based on the RT-qPCR method performed in samples of spleen, liver and small intestine we can state that MAP effectively infected only small intestine and the infection did not spread further. Moreover, IgY reduced the MAP levels in small intestine. After the decrease of MAP level in small intestine due to IgY addition, the IL-1β, IL-17, IFNγ and TNFα cytokine expression was measured. Unfortunately, the effect of IgY downregulating the expression of cytokines was not confirmed. Inflammatory cytokine modulation during infection by IgY was in some cases also detected in spleen and liver. However, MAP did not successfully infected this organs therefore it is probably the total response of mice to infection and the cause of this condition remains to be elucidated.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů