Characterisation of a ST100 Staphylococcus epidermidis producing an LnuB nucleotidyltransferase: Evidence for interspecies spread of an lnuB-carrying transposon

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F18%3AN0000120" target="_blank" >RIV/00027162:_____/18:N0000120 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11140/18:10373306 RIV/00216224:14310/18:00112624

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S2213716518300456?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S2213716518300456?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jgar.2018.02.017" target="_blank" >10.1016/j.jgar.2018.02.017</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Characterisation of a ST100 Staphylococcus epidermidis producing an LnuB nucleotidyltransferase: Evidence for interspecies spread of an lnuB-carrying transposon

Popis výsledku v původním jazyce

The increasing emergence of staphylococci resistant to lincosamides is a growing public health concern worldwide. Here, the structure of the first lsaE/lnuB-positive Staphylococcus epidermidis strain isolated in Greece is described. Multilocus sequence typing (MLST) classified the isolate as ST100. Sequence analysis showed that the strain harboured a 12.1-kb segment carrying lnuB and lsaE, flanked by insertion sequence IS257 in parallel orientation. Downstream of the left-hand IS257, four ORFs encoding a hypothetical protein, a putative methyltransferase, an amino-glycoside 6-adenylyltransferase (aadE) and an adenine phosphoribosyltransferase (apt) were found. The apt gene was followed by the spc gene conferring resistance to spectinomycin. A sequence comprising lsaE and lnuB and two ORFs encoding hypothetical proteins of unknown function was adjacent to the right-hand IS257. The lnuB-carrying segment shared extensive similarity (100% coverage, 99% identity) with the IS257-based composite transposon that was identified previously in ST9 methicillin-susceptible and ST398 methicillin-resistant Staphylococcus aureus of human origin. To our knowledge, this is the first description of an lnuB-positive ST100 S. epidermidis worldwide.

Název v anglickém jazyce

Characterisation of a ST100 Staphylococcus epidermidis producing an LnuB nucleotidyltransferase: Evidence for interspecies spread of an lnuB-carrying transposon

Popis výsledku anglicky

The increasing emergence of staphylococci resistant to lincosamides is a growing public health concern worldwide. Here, the structure of the first lsaE/lnuB-positive Staphylococcus epidermidis strain isolated in Greece is described. Multilocus sequence typing (MLST) classified the isolate as ST100. Sequence analysis showed that the strain harboured a 12.1-kb segment carrying lnuB and lsaE, flanked by insertion sequence IS257 in parallel orientation. Downstream of the left-hand IS257, four ORFs encoding a hypothetical protein, a putative methyltransferase, an amino-glycoside 6-adenylyltransferase (aadE) and an adenine phosphoribosyltransferase (apt) were found. The apt gene was followed by the spc gene conferring resistance to spectinomycin. A sequence comprising lsaE and lnuB and two ORFs encoding hypothetical proteins of unknown function was adjacent to the right-hand IS257. The lnuB-carrying segment shared extensive similarity (100% coverage, 99% identity) with the IS257-based composite transposon that was identified previously in ST9 methicillin-susceptible and ST398 methicillin-resistant Staphylococcus aureus of human origin. To our knowledge, this is the first description of an lnuB-positive ST100 S. epidermidis worldwide.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

30303 - Infectious Diseases

Projekt

<a href="/cs/project/NV17-29239A" target="_blank" >NV17-29239A: Klinické aspekty multirezistentních infekcí způsobených gramnegativními bakteriemi studované na klinicky relevantním modelu sepse</a><br>

Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Global Antimicrobial Resistance

ISSN

2213-7165

e-ISSN

2213-7173

Svazek periodika

13

Číslo periodika v rámci svazku

Jun

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

2

Strana od-do

9-10

Kód UT WoS článku

000438697700003

EID výsledku v databázi Scopus

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