Mannan-coated nanoliposome prepared via orthogonal aminooxy lipid-based click chemistry and microfluidics mixing: characterization of the structure and in vitro biological activities

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F18%3AN0000144" target="_blank" >RIV/00027162:_____/18:N0000144 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Mannan-coated nanoliposome prepared via orthogonal aminooxy lipid-based click chemistry and microfluidics mixing: characterization of the structure and in vitro biological activities

Popis výsledku v původním jazyce

Poster. New synthetic aminooxy lipid was used as building block for the formulation of functionalised nanoliposomes by microfluidic mixing. Outer surface of functionalised nanoliposomes was modified by orthogonal binding of reducing termini of cellular mannan (Candida glabrata (CCY 26-20-1) to oxime lipids via click chemistry reaction based on aminooxy coupling (oxime ligation). Aminooxy lipid was proved as suitable active component for preparation of functionalised nanoliposomes by the microfluidic mixing method. The structure of resulting mannan-liposomes were visualized by transmission (TEM) and scanning (SEM) electron microscopy as well as by dynamic light scattering (DLS), nanoparticle tracking analysis (NTA), and tunable resistive pulse sensing (TPRS). In vitro experiments on human and mouse dendritic cells demonstrate selective internalization of fluorochrome-labelled mannan-liposomes and their ability to stimulate DC comparable to lipopolysaccharide. We described a potentially new drug delivery platform for mannan receptor-targeted antimicrobial drugs as well as for immunotherapeutics. Furthermore, the platform based on mannans bound orthogonally onto the surface of nanoliposomes represents self-adjuvanted carrier for construction of liposome-based recombinant vaccines for both systemic and mucosal route of administration.

Název v anglickém jazyce

Mannan-coated nanoliposome prepared via orthogonal aminooxy lipid-based click chemistry and microfluidics mixing: characterization of the structure and in vitro biological activities

Popis výsledku anglicky

Poster. New synthetic aminooxy lipid was used as building block for the formulation of functionalised nanoliposomes by microfluidic mixing. Outer surface of functionalised nanoliposomes was modified by orthogonal binding of reducing termini of cellular mannan (Candida glabrata (CCY 26-20-1) to oxime lipids via click chemistry reaction based on aminooxy coupling (oxime ligation). Aminooxy lipid was proved as suitable active component for preparation of functionalised nanoliposomes by the microfluidic mixing method. The structure of resulting mannan-liposomes were visualized by transmission (TEM) and scanning (SEM) electron microscopy as well as by dynamic light scattering (DLS), nanoparticle tracking analysis (NTA), and tunable resistive pulse sensing (TPRS). In vitro experiments on human and mouse dendritic cells demonstrate selective internalization of fluorochrome-labelled mannan-liposomes and their ability to stimulate DC comparable to lipopolysaccharide. We described a potentially new drug delivery platform for mannan receptor-targeted antimicrobial drugs as well as for immunotherapeutics. Furthermore, the platform based on mannans bound orthogonally onto the surface of nanoliposomes represents self-adjuvanted carrier for construction of liposome-based recombinant vaccines for both systemic and mucosal route of administration.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

30104 - Pharmacology and pharmacy

Projekt

<a href="/cs/project/EF15_003%2F0000495" target="_blank" >EF15_003/0000495: FIT (Farmakologie, Imunoterapie, nanoToxikologie)</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů