Nucleoside inhibitors of tick-borne encephalitis virus: structure-activity relationships and drug resistance study

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F18%3AN0000147" target="_blank" >RIV/00027162:_____/18:N0000147 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Nucleoside inhibitors of tick-borne encephalitis virus: structure-activity relationships and drug resistance study

Popis výsledku v původním jazyce

THE THIRD BAIKAL INTERNATIONAL SCIENTIFIC CONFERENCE ACTIVITIES, Irkutsk, Russian federation, 27.-29. 9. 2018 -lecture.Tick-borne encephalitis virus (TBEV) represents one of the most serious arboviral neuro-infection in Europe and northern Asia. As no specific antiviral therapy is available at present, there is an urgent need for efficient drugs to treat patients with TBEV infection. We report here a structure-activity relationship study based on the antiviral/cytotoxicity profile of 29 nucleoside derivatives, each differing in chemical substituents on the ribose ring and in the type and chemical modifications of the heterobase. In order to assess the acquired resistance of TBEV to 2´-C-methyl modified nucleosides, we isolated and characterized a drug-resistant TBEV mutant by serial in vitro passage of the Hypr TBEV strain on porcine stable kidney cells under the selective pressure of 7-deaza-2´-C-methyladenosine.

Název v anglickém jazyce

Nucleoside inhibitors of tick-borne encephalitis virus: structure-activity relationships and drug resistance study

Popis výsledku anglicky

THE THIRD BAIKAL INTERNATIONAL SCIENTIFIC CONFERENCE ACTIVITIES, Irkutsk, Russian federation, 27.-29. 9. 2018 -lecture.Tick-borne encephalitis virus (TBEV) represents one of the most serious arboviral neuro-infection in Europe and northern Asia. As no specific antiviral therapy is available at present, there is an urgent need for efficient drugs to treat patients with TBEV infection. We report here a structure-activity relationship study based on the antiviral/cytotoxicity profile of 29 nucleoside derivatives, each differing in chemical substituents on the ribose ring and in the type and chemical modifications of the heterobase. In order to assess the acquired resistance of TBEV to 2´-C-methyl modified nucleosides, we isolated and characterized a drug-resistant TBEV mutant by serial in vitro passage of the Hypr TBEV strain on porcine stable kidney cells under the selective pressure of 7-deaza-2´-C-methyladenosine.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10607 - Virology

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů