Occurrence of gram-positive mastitis pathogens and their antimicrobial susceptibility in Czech dairy herds

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F18%3AN0000185" target="_blank" >RIV/00027162:_____/18:N0000185 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.bcva.eu/" target="_blank" >https://www.bcva.eu/</a>

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Occurrence of gram-positive mastitis pathogens and their antimicrobial susceptibility in Czech dairy herds

Popis výsledku v původním jazyce

Cattle Practice OCTOBER 2018 Volume 26 Part 2 BCVA Congress 2018 Additional posters, p. 4, Jurys Inn Hinckley Island Hotel, Watling Street, Hinckley, LE10 3JA, Anglie, 18.–20. october 2018 – poster. A total of 3,719 quarter milk samples were collected from cows with clinical or subclinical mastitis. The samples were delivered by local veterinarians from 112 farms in the eastern part of the Czech Republic between January 1st, 2017 and June 30th, 2018 to the State Veterinary Institute Olomouc, Czech Republic. The samples were conventionally bacteriologically investigated. The isolated strains were subsequently confirmed by the phenotypic molecular mass spectrometry method, MALDI TOF MS. Selected isolates of the most frequently found G+ pathogens were tested for susceptibility to antimicrobial agents by the standardized microdilution method determining the minimum inhibitory concentrations (MICs) of antimicrobials according to international standardized methodologies. The MICs were determined for 163 isolates of Streptococcus (Str.) uberis, 70 isolates of S. aureus, 63 isolates of S. chromogenes, 48 S. haemolyticus isolates, and 25 isolates of Str. dysgalactiae. Mastitis pathogens were found in 47.1% of the milk samples i.e. in 1,752 out of 3,719. A total of 2,284 isolates were of G+ pathogens, of which 1,501 belonged to Staphylococcus or Streptococcus genus. Samples with no growth or contamination constituted 21.4% and 31.5% of all samples, respectively. Str. uberis was the most frequently isolated pathogen (in 17.4% milk samples; 28.4% of G+ isolates), followed by S. aureus (11.7%; 19.1%) and coagulase-negative staphylococci (CNS; 9.6%; 15.6%). Str. dysgalactiae (1.1%; 1.8%) and Str. agalactiae (0.5%; 0.8%) were markedly less frequent. Susceptibility to antimicrobials varied among the five tested G+ mastitis species. No resistance was found to trimetoprim/sulfamethoxazole except for one isolate of S. chromogenes. Resistance to clindamycin, streptomycin and tetracycline were recorded across all tested species. Most of the resistant isolates were found in S. aureus. The S. aureus isolates were to varying degrees resistant to almost all tested antimicrobials (penicillin 41.4%; tetracycline 28.6%; cefoxitin 25.7%; gentamicin 15.7%; amoxicillin/clavulanic acid 14.3%; oxacillin 12.9%; ceftiofur 11.4%; streptomycin 7.1%; clindamycin 2.9%; neomycin 1.4%; enrofloxacin 1.4%; rifampicin 1.4%). The only exception was trimetoprim/sulfamethoxazole, to which all isolates were susceptible. Intermediate isolates of S. aureus were detected to ceftiofur (11.4%), clindamycin (5.7%), streptomycin (4.3%), neomycin (2.9%), and rifampicin (2.9%). Moreover, based on the occurrence of isolates resistant to oxacillin and cefoxitin, some isolates of S. aureus could be methicillin resistant. Similarly, the occurrence of resistance to all of the above antimicrobials, with the exception of oxacillin or trimethoprim, was found in the isolates of S. chromogenes and S. haemolyticus, mostly to a lesser extent. Their resistance was 17.5% and 35.5 %, 9.5% and 8.3%, 28.6% and 10.4%, to penicillin, streptomycin and tetracycline, respectively. In the Str. uberis and Str. dysgalactiae isolates, resistance was observed in 30.1% and 12%, 52.1% and 28%, 63.2% and 60% to clindamycin, streptomycin and tetracycline, respectively. Only in Str. dysgalactiae, a 100% susceptibility to β-lactams antimicrobials was reported. In St. uberis, 35 % of intermediate isolates to penicillin, 63.2% to rifampicin, and 2.5% to enrofloxacin and ceftiofur were detected.

Název v anglickém jazyce

Occurrence of gram-positive mastitis pathogens and their antimicrobial susceptibility in Czech dairy herds

Popis výsledku anglicky

Cattle Practice OCTOBER 2018 Volume 26 Part 2 BCVA Congress 2018 Additional posters, p. 4, Jurys Inn Hinckley Island Hotel, Watling Street, Hinckley, LE10 3JA, Anglie, 18.–20. october 2018 – poster. A total of 3,719 quarter milk samples were collected from cows with clinical or subclinical mastitis. The samples were delivered by local veterinarians from 112 farms in the eastern part of the Czech Republic between January 1st, 2017 and June 30th, 2018 to the State Veterinary Institute Olomouc, Czech Republic. The samples were conventionally bacteriologically investigated. The isolated strains were subsequently confirmed by the phenotypic molecular mass spectrometry method, MALDI TOF MS. Selected isolates of the most frequently found G+ pathogens were tested for susceptibility to antimicrobial agents by the standardized microdilution method determining the minimum inhibitory concentrations (MICs) of antimicrobials according to international standardized methodologies. The MICs were determined for 163 isolates of Streptococcus (Str.) uberis, 70 isolates of S. aureus, 63 isolates of S. chromogenes, 48 S. haemolyticus isolates, and 25 isolates of Str. dysgalactiae. Mastitis pathogens were found in 47.1% of the milk samples i.e. in 1,752 out of 3,719. A total of 2,284 isolates were of G+ pathogens, of which 1,501 belonged to Staphylococcus or Streptococcus genus. Samples with no growth or contamination constituted 21.4% and 31.5% of all samples, respectively. Str. uberis was the most frequently isolated pathogen (in 17.4% milk samples; 28.4% of G+ isolates), followed by S. aureus (11.7%; 19.1%) and coagulase-negative staphylococci (CNS; 9.6%; 15.6%). Str. dysgalactiae (1.1%; 1.8%) and Str. agalactiae (0.5%; 0.8%) were markedly less frequent. Susceptibility to antimicrobials varied among the five tested G+ mastitis species. No resistance was found to trimetoprim/sulfamethoxazole except for one isolate of S. chromogenes. Resistance to clindamycin, streptomycin and tetracycline were recorded across all tested species. Most of the resistant isolates were found in S. aureus. The S. aureus isolates were to varying degrees resistant to almost all tested antimicrobials (penicillin 41.4%; tetracycline 28.6%; cefoxitin 25.7%; gentamicin 15.7%; amoxicillin/clavulanic acid 14.3%; oxacillin 12.9%; ceftiofur 11.4%; streptomycin 7.1%; clindamycin 2.9%; neomycin 1.4%; enrofloxacin 1.4%; rifampicin 1.4%). The only exception was trimetoprim/sulfamethoxazole, to which all isolates were susceptible. Intermediate isolates of S. aureus were detected to ceftiofur (11.4%), clindamycin (5.7%), streptomycin (4.3%), neomycin (2.9%), and rifampicin (2.9%). Moreover, based on the occurrence of isolates resistant to oxacillin and cefoxitin, some isolates of S. aureus could be methicillin resistant. Similarly, the occurrence of resistance to all of the above antimicrobials, with the exception of oxacillin or trimethoprim, was found in the isolates of S. chromogenes and S. haemolyticus, mostly to a lesser extent. Their resistance was 17.5% and 35.5 %, 9.5% and 8.3%, 28.6% and 10.4%, to penicillin, streptomycin and tetracycline, respectively. In the Str. uberis and Str. dysgalactiae isolates, resistance was observed in 30.1% and 12%, 52.1% and 28%, 63.2% and 60% to clindamycin, streptomycin and tetracycline, respectively. Only in Str. dysgalactiae, a 100% susceptibility to β-lactams antimicrobials was reported. In St. uberis, 35 % of intermediate isolates to penicillin, 63.2% to rifampicin, and 2.5% to enrofloxacin and ceftiofur were detected.

Druh

O - Ostatní výsledky

CEP obor

—

OECD FORD obor

40301 - Veterinary science

Projekt

<a href="/cs/project/QJ1510217" target="_blank" >QJ1510217: Návrh a uplatnění plošného systému sběru dat o nemocech skotu a jeho využití v managementu stád, šlechtění a pro racionální užívání antimikrobik</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů