Preparation of cryosections from frozen porcine pulmonary tissue for MALDI mass spectrometry imaging

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F18%3AN0000193" target="_blank" >RIV/00027162:_____/18:N0000193 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62156489:43210/18:43914712 RIV/00216305:26620/19:PU131634

Výsledek na webu

<a href="https://mnet.mendelu.cz/mendelnet2018/mnet_2018_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2018/mnet_2018_full.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Preparation of cryosections from frozen porcine pulmonary tissue for MALDI mass spectrometry imaging

Popis výsledku v původním jazyce

In last decades, a matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) was used for mapping the spatial distribution of different molecules, mainly peptides, proteins, lipids and metabolites, in different types of tissue. Cryosections are the best suited for getting as much information as possible because the preparation of samples for MALDI MSI does not include deparaffinization and antigen-retrieval steps which are needed in case of conventional formalin-fixed and paraffin-embedded (FFPE) tissue sections. To analyse infection markers like interleukins in the tissue sections of porcine lungs affected by Actinobacillus pleuropneumoniae, it was necessary to obtain very well prepared cryosections for MALDI MSI. Therefore, this work was focused on optimization of preparation of frozen porcine lungs and its cryosections with conserved morphological structure.

Název v anglickém jazyce

Preparation of cryosections from frozen porcine pulmonary tissue for MALDI mass spectrometry imaging

Popis výsledku anglicky

In last decades, a matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) was used for mapping the spatial distribution of different molecules, mainly peptides, proteins, lipids and metabolites, in different types of tissue. Cryosections are the best suited for getting as much information as possible because the preparation of samples for MALDI MSI does not include deparaffinization and antigen-retrieval steps which are needed in case of conventional formalin-fixed and paraffin-embedded (FFPE) tissue sections. To analyse infection markers like interleukins in the tissue sections of porcine lungs affected by Actinobacillus pleuropneumoniae, it was necessary to obtain very well prepared cryosections for MALDI MSI. Therefore, this work was focused on optimization of preparation of frozen porcine lungs and its cryosections with conserved morphological structure.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

<a href="/cs/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>

Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MendelNet 2018: Proceedings of 25th International PhD Students Conference

ISBN

978-80-7509-597-8

ISSN

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e-ISSN

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Počet stran výsledku

6

Strana od-do

506-511

Název nakladatele

Mendel University in Brno

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

7. 11. 2018

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

000462205300096