Optimalization of cryohistological technique in rat and porcine lungs

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F18%3AN0000194" target="_blank" >RIV/00027162:_____/18:N0000194 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62156489:43210/18:43914677

Výsledek na webu

<a href="https://mnet.mendelu.cz/mendelnet2018/mnet_2018_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2018/mnet_2018_full.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Optimalization of cryohistological technique in rat and porcine lungs

Popis výsledku v původním jazyce

Histological freezing technique is a quick and gentle method of tissue preparation for identifying the microscopic structure. Lung tissue belongs to the less compact structures and its proper histological processing required testing of several available procedures. The optimization of freezing pulmonary tissue leads to more accurate detection of the pathological process in the lungs and better understanding pathogenesis of respiratory diseases of pigs, especially the porcine pneumonia. The aim of this work was to find a way of preserving the morphological structure of the lungs as in a physiological state. We used pulmonary tissue from 4 laboratory rats and 4 domestic pigs and freezing of samples in liquid nitrogen, iso-pentane, n-heptane and the usual process in the freezer. The results show the most appropriate procedure was after isolation of the lungs, filled part of lung over the bronchus by mixture of 1 : 1 Tissue Tek (O.C.T. Compound) with phosphate-buffered saline solution (PBS) and frozen in super-cooled n-heptane placed on dry ice and samples store at -80 °C.

Název v anglickém jazyce

Optimalization of cryohistological technique in rat and porcine lungs

Popis výsledku anglicky

Histological freezing technique is a quick and gentle method of tissue preparation for identifying the microscopic structure. Lung tissue belongs to the less compact structures and its proper histological processing required testing of several available procedures. The optimization of freezing pulmonary tissue leads to more accurate detection of the pathological process in the lungs and better understanding pathogenesis of respiratory diseases of pigs, especially the porcine pneumonia. The aim of this work was to find a way of preserving the morphological structure of the lungs as in a physiological state. We used pulmonary tissue from 4 laboratory rats and 4 domestic pigs and freezing of samples in liquid nitrogen, iso-pentane, n-heptane and the usual process in the freezer. The results show the most appropriate procedure was after isolation of the lungs, filled part of lung over the bronchus by mixture of 1 : 1 Tissue Tek (O.C.T. Compound) with phosphate-buffered saline solution (PBS) and frozen in super-cooled n-heptane placed on dry ice and samples store at -80 °C.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

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Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MendelNet 2018: Proceedings of 25th International PhD Students Conference

ISBN

978-80-7509-597-8

ISSN

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e-ISSN

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Počet stran výsledku

5

Strana od-do

361-365

Název nakladatele

Mendel University in Brno

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

7. 11. 2018

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

000462205300067