Multiple virus infections in Western honeybee (Apis mellifera L.) ejaculate used for instrumental insemination

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F19%3AN0000006" target="_blank" >RIV/00027162:_____/19:N0000006 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60460709:41210/20:80497

Výsledek na webu

<a href="https://www.mdpi.com/1999-4915/11/4/306" target="_blank" >https://www.mdpi.com/1999-4915/11/4/306</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/v11040306" target="_blank" >10.3390/v11040306</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Multiple virus infections in Western honeybee (Apis mellifera L.) ejaculate used for instrumental insemination

Popis výsledku v původním jazyce

Instrumental insemination of Apis mellifera L. queens is widely employed technique used in honeybee breeding which enables effective control of mating. However, drone semen represents potential source of honeybee viruses. In this study, 43 semen doses collected from apparently healthy drones, and consequently used in instrumental insemination, were analysed using PCR or RT-PCR to detect the presence of viral genome of eleven honeybee viruses. In 90.7% of samples, viral infection was detected. The survey revealed genomes of five viruses, namely Deformed wing virus (DWV), Acute bee paralysis virus (ABPV), Black queen cell virus (BQCV), Sacbrood virus (SBV), and A. mellifera filamentous virus (AmFV) in 83.7%, 18.6%, 13.9%, 2.3%, and 67.4% of samples, respectively. Single infection (30.2% of samples) as well as multiple infection (60.5% of samples) of two, three or four pathogens were also evaluated. To the best of our knowledge, this is the first study describing the presence of the BQCV and SBV genome sequence in drone ejaculate. Phylogenetic analysis of BQCV partial helicase gene sequence revealed high similarity of nucleotide sequence of described Czech strains, which varied from 91.4% to 99.6%. The findings of our study indicate the possibility of venereal transmission of BQCV and SBV.

Název v anglickém jazyce

Multiple virus infections in Western honeybee (Apis mellifera L.) ejaculate used for instrumental insemination

Popis výsledku anglicky

Instrumental insemination of Apis mellifera L. queens is widely employed technique used in honeybee breeding which enables effective control of mating. However, drone semen represents potential source of honeybee viruses. In this study, 43 semen doses collected from apparently healthy drones, and consequently used in instrumental insemination, were analysed using PCR or RT-PCR to detect the presence of viral genome of eleven honeybee viruses. In 90.7% of samples, viral infection was detected. The survey revealed genomes of five viruses, namely Deformed wing virus (DWV), Acute bee paralysis virus (ABPV), Black queen cell virus (BQCV), Sacbrood virus (SBV), and A. mellifera filamentous virus (AmFV) in 83.7%, 18.6%, 13.9%, 2.3%, and 67.4% of samples, respectively. Single infection (30.2% of samples) as well as multiple infection (60.5% of samples) of two, three or four pathogens were also evaluated. To the best of our knowledge, this is the first study describing the presence of the BQCV and SBV genome sequence in drone ejaculate. Phylogenetic analysis of BQCV partial helicase gene sequence revealed high similarity of nucleotide sequence of described Czech strains, which varied from 91.4% to 99.6%. The findings of our study indicate the possibility of venereal transmission of BQCV and SBV.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40301 - Veterinary science

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Viruses

ISSN

1999-4915

e-ISSN

—

Svazek periodika

11

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

8

Strana od-do

306

Kód UT WoS článku

000467310600003

EID výsledku v databázi Scopus

2-s2.0-85064209954