Susceptibility testing of lactic acid bacteria used in dairy industry to slected antimicrobial agents

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F19%3AN0000047" target="_blank" >RIV/00027162:_____/19:N0000047 - isvavai.cz</a>

Výsledek na webu

<a href="http://hygiena-alimentorum.uvlf.sk/program.php" target="_blank" >http://hygiena-alimentorum.uvlf.sk/program.php</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Susceptibility testing of lactic acid bacteria used in dairy industry to slected antimicrobial agents

Popis výsledku v původním jazyce

Hygiena Alimentorum, 15.-17.5.2019, Štrbské Pleso (Slovakia) – lecture and text in peer-reviewed proceedings. Lactic acid bacteria (LAB) are widely used in food, feed and pharmaceutical industry. In order to avoid the spread of antibiotic resistance, bacteria introduced into food or feed should be tested for susceptibility to selected antimicrobial agents, and resistant strains associated with gene transfer should not be used. In this survey, we analysed 32 isolates of lactic acid bacteria and Propionibacterium spp. originating from culture collections of dairy microorganisms from two companies, using the broth microdilution method. Simultaneously, 20 isolates were also analysed using antimicrobial gradient strips (M.I.C.Evaluator strips). Five isolates showing resistance to aminoglycoside antibiotics and resistance to other studied antimicrobial agents were subjected to whole genome sequencing and based on the obtained data, they were analysed for transferable resistance genes. In total, 72% (23/32) isolates were phenotypically resistant to aminoglycoside antibiotics (streptomycin, kanamycin or gentamicin), and four of these isolates displayed simultaneous resistance to other five antibiotic agents, such as ampicillin, vancomycin, erythromycin, clindamycin or tetracycline. Using molecular analysis, a potentially transferable antibiotic resistance gene for aminoglycoside nucleotidyltransferase was confirmed in only one isolate.

Název v anglickém jazyce

Susceptibility testing of lactic acid bacteria used in dairy industry to slected antimicrobial agents

Popis výsledku anglicky

Hygiena Alimentorum, 15.-17.5.2019, Štrbské Pleso (Slovakia) – lecture and text in peer-reviewed proceedings. Lactic acid bacteria (LAB) are widely used in food, feed and pharmaceutical industry. In order to avoid the spread of antibiotic resistance, bacteria introduced into food or feed should be tested for susceptibility to selected antimicrobial agents, and resistant strains associated with gene transfer should not be used. In this survey, we analysed 32 isolates of lactic acid bacteria and Propionibacterium spp. originating from culture collections of dairy microorganisms from two companies, using the broth microdilution method. Simultaneously, 20 isolates were also analysed using antimicrobial gradient strips (M.I.C.Evaluator strips). Five isolates showing resistance to aminoglycoside antibiotics and resistance to other studied antimicrobial agents were subjected to whole genome sequencing and based on the obtained data, they were analysed for transferable resistance genes. In total, 72% (23/32) isolates were phenotypically resistant to aminoglycoside antibiotics (streptomycin, kanamycin or gentamicin), and four of these isolates displayed simultaneous resistance to other five antibiotic agents, such as ampicillin, vancomycin, erythromycin, clindamycin or tetracycline. Using molecular analysis, a potentially transferable antibiotic resistance gene for aminoglycoside nucleotidyltransferase was confirmed in only one isolate.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40201 - Animal and dairy science; (Animal biotechnology to be 4.4)

Projekt

<a href="/cs/project/QJ1510338" target="_blank" >QJ1510338: Fermentované mléčné výrobky a sýry pro zdravou výživu obyvatel, technologické postupy jejich výroby a metody hodnocení s důrazem na vysokou mikrobiologickou bezpečnost a zlepšené nutriční parametry</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů