Immune response of Koi carp and Amur wild carp infected with KHV

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F19%3AN0000213" target="_blank" >RIV/00027162:_____/19:N0000213 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Immune response of Koi carp and Amur wild carp infected with KHV

Popis výsledku v původním jazyce

19th International Conference on Diseases of Fish and Shellfish, Porto - Portugal, 9th-12th September 2019. Introduction: Koi herpesvirus (KHV) causes serious disease (KHVD) affecting common carp (Cyprinus carpio L.) and some of its hybrids with other members of Cyprinidae family. KHV mortality rates are influenced, besides environmental factors, age and health conditions, by carp strain, and may reach 100%, but also a couple of percents. Influence of KHV infection on expression levels of cytokines and other genes affecting immune system of carp was tested for revealing its possible influence on KHV mortality. Methodology: Two subspecies of common carp were chosen for KHV infection: Koi carp (C. c. “Koi”), which is very susceptible to the disease and Amur wild carp (C. c. haematopterus), strain resistant to diseases including KHVD. Carps of both subspecies were divided into two groups: control and infected. The virus was administered intraperitoneally with dose corresponding 104 TCID50/ml. Samples of blood, skin, gills, head kidney, spleen, hepatopancreas and gut were collected at two time points: 3 dpi and 7 dpi. Time points were chosen to record early immune response, between first clinical signs and early mortality. Expression of several immune reaction related genes were detected using qPCR method. The study was focused on pro-inflammatory cytokine IL1β, anti-inflammatory cytokine IL10 and some members of antiviral signaling pathway of class I IFN. Results: Symptoms of KHV were not detected in infected Amur wild carps. On the contrary in infected Koi carps was observed increased excretion of mucus at time point 7dpi, but no more symptoms of the disease. According to present data, the induction of expression of Il10 was detected in skin of infected Amur wild carps in contrast to its suppression in infected koi carp. Significantly higher expression of il1β was detected within gills and skin of Koi. Meanwhile there were not observed differences between levels of expressions of chosen members of IFN I signaling pathway. Conclusion: Obtained data shown difference between intensity of immune reaction of Koi carp and Amur wild carp on KHV infection, but so far there was no observed reason of lesser susceptibility of Amur wild carp to KHV.

Název v anglickém jazyce

Immune response of Koi carp and Amur wild carp infected with KHV

Popis výsledku anglicky

19th International Conference on Diseases of Fish and Shellfish, Porto - Portugal, 9th-12th September 2019. Introduction: Koi herpesvirus (KHV) causes serious disease (KHVD) affecting common carp (Cyprinus carpio L.) and some of its hybrids with other members of Cyprinidae family. KHV mortality rates are influenced, besides environmental factors, age and health conditions, by carp strain, and may reach 100%, but also a couple of percents. Influence of KHV infection on expression levels of cytokines and other genes affecting immune system of carp was tested for revealing its possible influence on KHV mortality. Methodology: Two subspecies of common carp were chosen for KHV infection: Koi carp (C. c. “Koi”), which is very susceptible to the disease and Amur wild carp (C. c. haematopterus), strain resistant to diseases including KHVD. Carps of both subspecies were divided into two groups: control and infected. The virus was administered intraperitoneally with dose corresponding 104 TCID50/ml. Samples of blood, skin, gills, head kidney, spleen, hepatopancreas and gut were collected at two time points: 3 dpi and 7 dpi. Time points were chosen to record early immune response, between first clinical signs and early mortality. Expression of several immune reaction related genes were detected using qPCR method. The study was focused on pro-inflammatory cytokine IL1β, anti-inflammatory cytokine IL10 and some members of antiviral signaling pathway of class I IFN. Results: Symptoms of KHV were not detected in infected Amur wild carps. On the contrary in infected Koi carps was observed increased excretion of mucus at time point 7dpi, but no more symptoms of the disease. According to present data, the induction of expression of Il10 was detected in skin of infected Amur wild carps in contrast to its suppression in infected koi carp. Significantly higher expression of il1β was detected within gills and skin of Koi. Meanwhile there were not observed differences between levels of expressions of chosen members of IFN I signaling pathway. Conclusion: Obtained data shown difference between intensity of immune reaction of Koi carp and Amur wild carp on KHV infection, but so far there was no observed reason of lesser susceptibility of Amur wild carp to KHV.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

<a href="/cs/project/EF16_019%2F0000869" target="_blank" >EF16_019/0000869: Udržitelná produkce zdravých ryb v různých akvakulturních systémech - PROFISH</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů