Comparative study of the bush dog (Speothos venaticus) karyotype and analysis of satellite DNA sequences and their chromosome distribution in six species of Canidae

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F19%3AN0000219" target="_blank" >RIV/00027162:_____/19:N0000219 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.karger.com/Article/Abstract/503082" target="_blank" >https://www.karger.com/Article/Abstract/503082</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1159/000503082" target="_blank" >10.1159/000503082</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Comparative study of the bush dog (Speothos venaticus) karyotype and analysis of satellite DNA sequences and their chromosome distribution in six species of Canidae

Popis výsledku v původním jazyce

The bush dog (Speothos venaticus, 2n=74) is a near threatened species taxonomically classified among South American canids. We revised the bush dog karyotype, and performed a comparative sequence analysis of satellite and satellite-like DNAs in six canids: the bush dog, domestic dog (Canis familiaris, 2n=78), grey wolf (Canis lupus, 2n=78), Chinese raccoon dog (Nyctereutes procyonoides procyonoides, 2n=54+B), red fox (Vulpes vulpes, 2n=34+B) and arctic fox (Vulpes lagopus, 2n=48-50) to specify the species position among Canidae. Using fluorescence in situ hybridisation with painting and BAC probes, we found that the distribution of canid evolutionarily conserved chromosome segments in the bush dog karyotype is similar to that of the domestic dog and grey wolf. The bush dog karyotype differs by two acrocentric chromosome pairs formed by tandem fusions of the canine (29;34) and (26;35) orthologues. An interstitial signal of the telomeric probe was observed in the (26;35) fusion site in the bush dog indicating a recent evolutionary origin of this rearrangement. Sequences and hybridisation patterns of satellite DNAs were compared, and a phylogenetic tree of the six canid species was constructed which confirmed the bush dog position close to the wolf-like canids, and apart from the raccoon dog and foxes.

Název v anglickém jazyce

Comparative study of the bush dog (Speothos venaticus) karyotype and analysis of satellite DNA sequences and their chromosome distribution in six species of Canidae

Popis výsledku anglicky

The bush dog (Speothos venaticus, 2n=74) is a near threatened species taxonomically classified among South American canids. We revised the bush dog karyotype, and performed a comparative sequence analysis of satellite and satellite-like DNAs in six canids: the bush dog, domestic dog (Canis familiaris, 2n=78), grey wolf (Canis lupus, 2n=78), Chinese raccoon dog (Nyctereutes procyonoides procyonoides, 2n=54+B), red fox (Vulpes vulpes, 2n=34+B) and arctic fox (Vulpes lagopus, 2n=48-50) to specify the species position among Canidae. Using fluorescence in situ hybridisation with painting and BAC probes, we found that the distribution of canid evolutionarily conserved chromosome segments in the bush dog karyotype is similar to that of the domestic dog and grey wolf. The bush dog karyotype differs by two acrocentric chromosome pairs formed by tandem fusions of the canine (29;34) and (26;35) orthologues. An interstitial signal of the telomeric probe was observed in the (26;35) fusion site in the bush dog indicating a recent evolutionary origin of this rearrangement. Sequences and hybridisation patterns of satellite DNAs were compared, and a phylogenetic tree of the six canid species was constructed which confirmed the bush dog position close to the wolf-like canids, and apart from the raccoon dog and foxes.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10603 - Genetics and heredity (medical genetics to be 3)

Projekt

<a href="/cs/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cytogenetic and Genome Research

ISSN

1424-8581

e-ISSN

1424-859X

Svazek periodika

—

Číslo periodika v rámci svazku

9.10.2019

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

9

Strana od-do

88-96

Kód UT WoS článku

000505202200005

EID výsledku v databázi Scopus

—