Gene Expression and Immunological Response in Mice Exposed to ZnO Nanoparticles
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F19%3AN0000247" target="_blank" >RIV/00027162:_____/19:N0000247 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/68081715:_____/18:00499201 RIV/68378041:_____/18:00499201 RIV/67985904:_____/18:00499201 RIV/61989100:27200/18:10242335
Výsledek na webu
<a href="https://asep.lib.cas.cz/arl-cav/cs/detail-cav_un_epca-0499201-GENE-EXPRESSION-AND-IMMUNOLOGICAL-RESPONSE-IN-MICE-EXPOSED-TO-ZnO-NANOPARTICLES/" target="_blank" >https://asep.lib.cas.cz/arl-cav/cs/detail-cav_un_epca-0499201-GENE-EXPRESSION-AND-IMMUNOLOGICAL-RESPONSE-IN-MICE-EXPOSED-TO-ZnO-NANOPARTICLES/</a>
DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Gene Expression and Immunological Response in Mice Exposed to ZnO Nanoparticles
Popis výsledku v původním jazyce
We analyzed gene expression changes in the lungs and the immunological response in splenocytes of mice exposed by inhalation of ZnO nanoparticles (NP). Adult female ICR mice were treated for three days and three months, respectively. Analysis of differential expression in genes involved in oxidative stress was conducted using quantitative RT-PCR. The potential immunotoxic and immunomodulatory effects of ZnO NP were analyzed by phenotyping and cytokine production by splenocytes after three months exposure. Three days exposure resulted in down-regulation of GCLC, GSR, HMOX-1, NQO-1, NF-kB2, PTGS2 and TXNRD1 mRNA expression; three months exposure increased the expression of these genes. Three months exposure caused a significant decrease in the percentage of granulocytes in the spleen cells, and affected the production of IL-10 and IL-6 by lipopolysaccharide-stimulated leukocytes. In summary, our study revealed changes in the expression of genes involved in the oxidative stress response following acute ZnO NP exposure. Subchronic ZnO NP exposure induced immunomodulatory effects in the spleen.
Název v anglickém jazyce
Gene Expression and Immunological Response in Mice Exposed to ZnO Nanoparticles
Popis výsledku anglicky
We analyzed gene expression changes in the lungs and the immunological response in splenocytes of mice exposed by inhalation of ZnO nanoparticles (NP). Adult female ICR mice were treated for three days and three months, respectively. Analysis of differential expression in genes involved in oxidative stress was conducted using quantitative RT-PCR. The potential immunotoxic and immunomodulatory effects of ZnO NP were analyzed by phenotyping and cytokine production by splenocytes after three months exposure. Three days exposure resulted in down-regulation of GCLC, GSR, HMOX-1, NQO-1, NF-kB2, PTGS2 and TXNRD1 mRNA expression; three months exposure increased the expression of these genes. Three months exposure caused a significant decrease in the percentage of granulocytes in the spleen cells, and affected the production of IL-10 and IL-6 by lipopolysaccharide-stimulated leukocytes. In summary, our study revealed changes in the expression of genes involved in the oxidative stress response following acute ZnO NP exposure. Subchronic ZnO NP exposure induced immunomodulatory effects in the spleen.
Klasifikace
Druh
D - Stať ve sborníku
CEP obor
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OECD FORD obor
30108 - Toxicology
Návaznosti výsledku
Projekt
<a href="/cs/project/GBP503%2F12%2FG147" target="_blank" >GBP503/12/G147: Centrum studií toxických vlastností nanočástic</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2019
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název statě ve sborníku
9th International Conference on Nanomaterials - Research and Application (NANOCON 2017)
ISBN
978-80-87294-81-9
ISSN
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e-ISSN
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Počet stran výsledku
6
Strana od-do
650-655
Název nakladatele
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Místo vydání
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Místo konání akce
Brno
Datum konání akce
18. 10. 2017
Typ akce podle státní příslušnosti
WRD - Celosvětová akce
Kód UT WoS článku
000452823300107