První průkaz a charakterizace viru porcinní hemaglutinující encefalomyelitidy v České republice

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F19%3AN0000340" target="_blank" >RIV/00027162:_____/19:N0000340 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.agriculturejournals.cz/web/vetmed.htm?type=article&id=95_2018-VETMED" target="_blank" >https://www.agriculturejournals.cz/web/vetmed.htm?type=article&id=95_2018-VETMED</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17221/95/2018-VETMED" target="_blank" >10.17221/95/2018-VETMED</a>

Jazyk výsledku

čeština

Název v původním jazyce

První průkaz a charakterizace viru porcinní hemaglutinující encefalomyelitidy v České republice

Popis výsledku v původním jazyce

Porcine hemagglutinating encephalomyelitis virus (PHEV) is a highly neurovirulent coronavirus that invades the central nervous system in piglets. The incidence of PHE among pigs in many countries is rising and the economic losses to the pig industry may be significant. According to the serological studies, it is assumed that PHEV is spread worldwide. However, no surveillance has been carried out in the Czech Republic. During this study eight pig farms were screened for the presence of members of Coronaviridae family with the use of reverse transcription PCR. The collection of 123 faecal samples and 151 nasal swabs from domestic pigs were analysed. In PHEV-positive samples almost complete coding sequence of nucleocapsid gene was amplified and the acquired sequences were compared to those of geographically different PHEV strains and phylogenetic analyses was performed. PHEV was present in 7.9% of nasal swabs taken from different age categories of pigs. No other swine coronaviruses were detected. The phylogeny showed 95.8-98.1% similarity in amino acid sequence of the Czech PHEV strains compared to other PHEV reference strains in GenBank. The PHEV strains collected from animals on the same farm were identical; however, strains from different farms have only 96.7-98.7% of amino acid sequence similarity. Our study demonstrated presence of PHEV in pigs in the Czech Republic. The Czech PHEV strains were evolutionary closest to the Belgium strain VW572.

Název v anglickém jazyce

First detection and characterization of porcine hemagglutinating encephalomyelitis virus in the Czech Republic

Popis výsledku anglicky

Porcine hemagglutinating encephalomyelitis virus (PHEV) is a highly neurovirulent coronavirus that invades the central nervous system in piglets. The incidence of PHE among pigs in many countries is rising and the economic losses to the pig industry may be significant. According to the serological studies, it is assumed that PHEV is spread worldwide. However, no surveillance has been carried out in the Czech Republic. During this study eight pig farms were screened for the presence of members of Coronaviridae family with the use of reverse transcription PCR. The collection of 123 faecal samples and 151 nasal swabs from domestic pigs were analysed. In PHEV-positive samples almost complete coding sequence of nucleocapsid gene was amplified and the acquired sequences were compared to those of geographically different PHEV strains and phylogenetic analyses was performed. PHEV was present in 7.9% of nasal swabs taken from different age categories of pigs. No other swine coronaviruses were detected. The phylogeny showed 95.8-98.1% similarity in amino acid sequence of the Czech PHEV strains compared to other PHEV reference strains in GenBank. The PHEV strains collected from animals on the same farm were identical; however, strains from different farms have only 96.7-98.7% of amino acid sequence similarity. Our study demonstrated presence of PHEV in pigs in the Czech Republic. The Czech PHEV strains were evolutionary closest to the Belgium strain VW572.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40301 - Veterinary science

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Veterinární medicína

ISSN

0375-8427

e-ISSN

1805-9392

Svazek periodika

64

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

7

Strana od-do

60-66

Kód UT WoS článku

000459023400002

EID výsledku v databázi Scopus

2-s2.0-85063731253