Subchronic continuous inhalation exposure to zinc oxide nanoparticles induces pulmonary cell response in mice

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F20%3AN0000116" target="_blank" >RIV/00027162:_____/20:N0000116 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68081715:_____/20:00525577 RIV/67985858:_____/20:00525577 RIV/67985904:_____/20:00525577 RIV/00216224:14310/20:00116178

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0946672X20300766" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0946672X20300766</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jtemb.2020.126511" target="_blank" >10.1016/j.jtemb.2020.126511</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Subchronic continuous inhalation exposure to zinc oxide nanoparticles induces pulmonary cell response in mice

Popis výsledku v původním jazyce

Objectives: We used mice as an animal model to investigate the entry of ZnO nanoparticles from the ambient air into the lungs and other organs, subsequent changes in Zn levels and the impact on the transcription of Zn homeostasis-related genes in the lungs. Methods: The mice were exposed to two concentrations of ZnO nanoparticles; lower (6.46 x 10(4) particles/cm(3)) and higher (1.93 x 10(6) particles/cm(3)), allowed to breathe the nanoparticles in the air for 12 weeks and subjected to necropsy. Characterization of the ZnO nanoparticles was done using transmission electron microscopy (TEM). Energy-dispersive X-ray (EDX) spectroscopy was used to quantify ZnO nanoparticles in the lungs, brain, liver and kidney. The total zinc content in the lungs, brain, liver, kidney, red blood cells and plasma was estimated by inductively coupled plasma mass spectroscopy (ICP-MS). Transcription rate of the genes was evaluated by RealTime PCR. Results: The two concentration of ZnO nanoparticles in the ambient air produced two different outcomes. The lower concentration resulted in significant increases in Zn content of the liver while the higher concentration significantly increased Zn in the lungs (p < 0.05). Additionally, at the lower concentration, Zn content was found to be lower in brain tissue (p < 0.05). Using TEM/EDX we detected ZnO nanoparticles inside the cells in the lungs, kidney and liver. Inhaling ZnO NP at the higher concentration increased the levels of mRNA of the following genes in the lungs: Mt2 (2.56 fold), Slc30a1 (1.52 fold) and S1c30a5 (2.34 fold). At the lower ZnO nanoparticle concentration, only S1c30a7 mRNA levels in the lungs were up (1.74 fold). Thus the two air concentrations of ZnO nanoparticles produced distinct effects on the expression of the Zn-homeostasis related genes.

Název v anglickém jazyce

Subchronic continuous inhalation exposure to zinc oxide nanoparticles induces pulmonary cell response in mice

Popis výsledku anglicky

Objectives: We used mice as an animal model to investigate the entry of ZnO nanoparticles from the ambient air into the lungs and other organs, subsequent changes in Zn levels and the impact on the transcription of Zn homeostasis-related genes in the lungs. Methods: The mice were exposed to two concentrations of ZnO nanoparticles; lower (6.46 x 10(4) particles/cm(3)) and higher (1.93 x 10(6) particles/cm(3)), allowed to breathe the nanoparticles in the air for 12 weeks and subjected to necropsy. Characterization of the ZnO nanoparticles was done using transmission electron microscopy (TEM). Energy-dispersive X-ray (EDX) spectroscopy was used to quantify ZnO nanoparticles in the lungs, brain, liver and kidney. The total zinc content in the lungs, brain, liver, kidney, red blood cells and plasma was estimated by inductively coupled plasma mass spectroscopy (ICP-MS). Transcription rate of the genes was evaluated by RealTime PCR. Results: The two concentration of ZnO nanoparticles in the ambient air produced two different outcomes. The lower concentration resulted in significant increases in Zn content of the liver while the higher concentration significantly increased Zn in the lungs (p < 0.05). Additionally, at the lower concentration, Zn content was found to be lower in brain tissue (p < 0.05). Using TEM/EDX we detected ZnO nanoparticles inside the cells in the lungs, kidney and liver. Inhaling ZnO NP at the higher concentration increased the levels of mRNA of the following genes in the lungs: Mt2 (2.56 fold), Slc30a1 (1.52 fold) and S1c30a5 (2.34 fold). At the lower ZnO nanoparticle concentration, only S1c30a7 mRNA levels in the lungs were up (1.74 fold). Thus the two air concentrations of ZnO nanoparticles produced distinct effects on the expression of the Zn-homeostasis related genes.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

21001 - Nano-materials (production and properties)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY

ISSN

0946-672X

e-ISSN

—

Svazek periodika

61

Číslo periodika v rámci svazku

Sep 2020

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

10

Strana od-do

"126511"

Kód UT WoS článku

000543366700011

EID výsledku v databázi Scopus

2-s2.0-85083016978