Development and inter-laboratory validation of diagnostics panel for detection of biothreat bacteria based on MOL-PCR assay

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F21%3AN0000001" target="_blank" >RIV/00027162:_____/21:N0000001 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60162694:G32__/21:N0000002 RIV/60162694:G33__/20:N0000007 RIV/62157124:16270/21:43879157 RIV/60162694:_____/21:N0000010 RIV/00216224:14310/21:00120920

Výsledek na webu

<a href="https://www.mdpi.com/2076-2607/9/1/38" target="_blank" >https://www.mdpi.com/2076-2607/9/1/38</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/microorganisms9010038" target="_blank" >10.3390/microorganisms9010038</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Development and inter-laboratory validation of diagnostics panel for detection of biothreat bacteria based on MOL-PCR assay

Popis výsledku v původním jazyce

Early detection of biohazardous bacteria that can be misused as biological weapons is one of the most important measures to prevent the spread and outbreak of biological warfare. The purpose of this study was to establish a new detection panel for biothreat bacteria (Bacillus anthracis, Yersinia pestis, Francisella tularensis and Brucella spp.) and confirm it by collaborative validation by using a multiplex oligonucleotide ligation followed by polymerase chain reaction and hybridization to microspheres by MagPix detection platform (MOL-PCR). The specific sequences in bacterial DNA were selected and MOLigo pair probes were designed. No cross-reactivity was recorded during testing between biothreat bacteria or using other selected unrelated bacterial agents. The sensitivities of these assays were at least 0.5 ng/µL for all target bacteria when serially diluted DNA was used as a template. When testing the freezing stability of ligation premixes, it was better to store MOLigo probes separately as probes 1 and probes 2. This highly multiplex method can be used for rapid screening to prevent outbreaks arising from the use of bacterial strains for bioterrorism.

Název v anglickém jazyce

Development and inter-laboratory validation of diagnostics panel for detection of biothreat bacteria based on MOL-PCR assay

Popis výsledku anglicky

Early detection of biohazardous bacteria that can be misused as biological weapons is one of the most important measures to prevent the spread and outbreak of biological warfare. The purpose of this study was to establish a new detection panel for biothreat bacteria (Bacillus anthracis, Yersinia pestis, Francisella tularensis and Brucella spp.) and confirm it by collaborative validation by using a multiplex oligonucleotide ligation followed by polymerase chain reaction and hybridization to microspheres by MagPix detection platform (MOL-PCR). The specific sequences in bacterial DNA were selected and MOLigo pair probes were designed. No cross-reactivity was recorded during testing between biothreat bacteria or using other selected unrelated bacterial agents. The sensitivities of these assays were at least 0.5 ng/µL for all target bacteria when serially diluted DNA was used as a template. When testing the freezing stability of ligation premixes, it was better to store MOLigo probes separately as probes 1 and probes 2. This highly multiplex method can be used for rapid screening to prevent outbreaks arising from the use of bacterial strains for bioterrorism.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Microorganism

ISSN

2076-2607

e-ISSN

2076-2607

Svazek periodika

9

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

15

Strana od-do

"38"

Kód UT WoS článku

000610579000001

EID výsledku v databázi Scopus

2-s2.0-85098765871