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The colonisation of Czech travellers and expatriates living in the Czech Republic by colistin-resistant Enterobacteriaceae and whole genome characterisation of E. coli isolates harbouring the mcr-1 genes on a plasmid or chromosome: A cross-sectional study

Identifikátory výsledku

  • Kód výsledku v IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F21%3AN0000010" target="_blank" >RIV/00027162:_____/21:N0000010 - isvavai.cz</a>

  • Nalezeny alternativní kódy

    RIV/00216208:11130/21:10417651 RIV/00064211:_____/21:W0000042 RIV/00064203:_____/21:10417651 RIV/00216224:14310/21:00120823

  • Výsledek na webu

    <a href="https://www.sciencedirect.com/science/article/pii/S1477893920304105?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1477893920304105?via%3Dihub</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.tmaid.2020.101914" target="_blank" >10.1016/j.tmaid.2020.101914</a>

Alternativní jazyky

  • Jazyk výsledku

    angličtina

  • Název v původním jazyce

    The colonisation of Czech travellers and expatriates living in the Czech Republic by colistin-resistant Enterobacteriaceae and whole genome characterisation of E. coli isolates harbouring the mcr-1 genes on a plasmid or chromosome: A cross-sectional study

  • Popis výsledku v původním jazyce

    Background: Travellers were recognized as risk cohort that can be colonized by mcr-1-mediated colistin resistant Enterobacteriaceae. We aimed to investigate the carriage of mcr-mediated colistin resistance in Enterobacteriaceae in Czech travellers or expatriates residing temporarily in the Czech Republic. Methods: Between August 2018 and September 2019, the stool samples were cultured in Enterobacteriaceae enrichment broth. The enriched cultures were tested for the presence of mcr-1-8 genes and inoculated onto selective agar with colistin. A minimal inhibitory concentration of colistin was determined by using the broth microdilution method. Colistin resistant Enterobacteriaceae isolates were tested for the presence of mcr-1-8 genes and mcr-positive isolates were characterised by whole genome sequencing. Results: From 177 stool samples, 15 Enterobacteriaceae isolates were cultured (7.9%); two E. coli isolates carried mcr-1 gene (1.1%). In E. coli sequence type (ST) 156, the mcr-1 gene was located in an ISApl1-mcr-1-orf-ISApl1 (Tn6330) and incorporated into the chromosome; in E. coli ST23 isolate, the mcr-1 gene was harboured by the plasmid IncX4. Both, mcr-1 positive E. coli were multidrug resistant and, in addition, one isolate was an extended-spectrum β-lactamase (ESBL) producer (blaCTX-M-27). Conclusion: Czech travellers, or expatriates in the Czech Republic, can be colonized by multidrug resistant Enterobacteriaceae harbouring the mcr-1 gene.

  • Název v anglickém jazyce

    The colonisation of Czech travellers and expatriates living in the Czech Republic by colistin-resistant Enterobacteriaceae and whole genome characterisation of E. coli isolates harbouring the mcr-1 genes on a plasmid or chromosome: A cross-sectional study

  • Popis výsledku anglicky

    Background: Travellers were recognized as risk cohort that can be colonized by mcr-1-mediated colistin resistant Enterobacteriaceae. We aimed to investigate the carriage of mcr-mediated colistin resistance in Enterobacteriaceae in Czech travellers or expatriates residing temporarily in the Czech Republic. Methods: Between August 2018 and September 2019, the stool samples were cultured in Enterobacteriaceae enrichment broth. The enriched cultures were tested for the presence of mcr-1-8 genes and inoculated onto selective agar with colistin. A minimal inhibitory concentration of colistin was determined by using the broth microdilution method. Colistin resistant Enterobacteriaceae isolates were tested for the presence of mcr-1-8 genes and mcr-positive isolates were characterised by whole genome sequencing. Results: From 177 stool samples, 15 Enterobacteriaceae isolates were cultured (7.9%); two E. coli isolates carried mcr-1 gene (1.1%). In E. coli sequence type (ST) 156, the mcr-1 gene was located in an ISApl1-mcr-1-orf-ISApl1 (Tn6330) and incorporated into the chromosome; in E. coli ST23 isolate, the mcr-1 gene was harboured by the plasmid IncX4. Both, mcr-1 positive E. coli were multidrug resistant and, in addition, one isolate was an extended-spectrum β-lactamase (ESBL) producer (blaCTX-M-27). Conclusion: Czech travellers, or expatriates in the Czech Republic, can be colonized by multidrug resistant Enterobacteriaceae harbouring the mcr-1 gene.

Klasifikace

  • Druh

    J<sub>imp</sub> - Článek v periodiku v databázi Web of Science

  • CEP obor

  • OECD FORD obor

    30303 - Infectious Diseases

Návaznosti výsledku

  • Projekt

    <a href="/cs/project/NV18-09-00254" target="_blank" >NV18-09-00254: Sentinelová studie výskytu na plazmid vázané (přenositelné) rezistence ke kolistinu u Enterobacteriaceae v České republice</a><br>

  • Návaznosti

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Ostatní

  • Rok uplatnění

    2021

  • Kód důvěrnosti údajů

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Údaje specifické pro druh výsledku

  • Název periodika

    Travel Medicine and Infectious Disease

  • ISSN

    1477-8939

  • e-ISSN

    1873-0442

  • Svazek periodika

    39

  • Číslo periodika v rámci svazku

    JAN 2021

  • Stát vydavatele periodika

    GB - Spojené království Velké Británie a Severního Irska

  • Počet stran výsledku

    5

  • Strana od-do

  • Kód UT WoS článku

    000620092200007

  • EID výsledku v databázi Scopus

    2-s2.0-85096467826