Development of piglet gut microbiota at the time of weaning influences development of postweaning diarrhea

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F21%3AN0000033" target="_blank" >RIV/00027162:_____/21:N0000033 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0034528820311243" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0034528820311243</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.rvsc.2020.12.022" target="_blank" >10.1016/j.rvsc.2020.12.022</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Development of piglet gut microbiota at the time of weaning influences development of postweaning diarrhea

Popis výsledku v původním jazyce

Postweaning diarrhea is a common issue in pig production which is currently controlled by feed supplementation with zinc oxide. However, new alternatives are being sought due to an expected ban on zinc oxide in feed supplementation from 2022 in the EU. One possible alternative is to use novel types of probiotics consisting of microbiota characteristic for healthy weaned piglets. In this study, we therefore collected rectal swabs of piglets 3 days before weaning and 4 days after weaning in a commercial farm considering all risks of field trial like the use of antibiotics, classified the piglets as predisposed, healthy or sick and using 16S rRNA sequencing, we determined and compared the microbiota composition. Increased Actinobacteria before weaning was a marker of piglets predisposed for diarrhea. Increased Chlamydia or Helicobacter before weaning was surprisingly a marker of healthy and resistant piglets after weaning. After weaning, unclassified Clostridiales, Deltaproteobacteria, Selenomonadales, Fusobacterium, Akkermansia or Anaerovibrio increased in microbiota of piglets with postweaning diarrhea while an increase in Prevotella and Faecalibacterium was characteristic for healthy, weaned piglets. Both changes in individual microbiota members and also correct timing of microbiota reshaping around weaning and the increase of mainly Prevotella species just after weaning are equally important for resistance to postweaning diarrhea in piglets under field conditions.

Název v anglickém jazyce

Development of piglet gut microbiota at the time of weaning influences development of postweaning diarrhea

Popis výsledku anglicky

Postweaning diarrhea is a common issue in pig production which is currently controlled by feed supplementation with zinc oxide. However, new alternatives are being sought due to an expected ban on zinc oxide in feed supplementation from 2022 in the EU. One possible alternative is to use novel types of probiotics consisting of microbiota characteristic for healthy weaned piglets. In this study, we therefore collected rectal swabs of piglets 3 days before weaning and 4 days after weaning in a commercial farm considering all risks of field trial like the use of antibiotics, classified the piglets as predisposed, healthy or sick and using 16S rRNA sequencing, we determined and compared the microbiota composition. Increased Actinobacteria before weaning was a marker of piglets predisposed for diarrhea. Increased Chlamydia or Helicobacter before weaning was surprisingly a marker of healthy and resistant piglets after weaning. After weaning, unclassified Clostridiales, Deltaproteobacteria, Selenomonadales, Fusobacterium, Akkermansia or Anaerovibrio increased in microbiota of piglets with postweaning diarrhea while an increase in Prevotella and Faecalibacterium was characteristic for healthy, weaned piglets. Both changes in individual microbiota members and also correct timing of microbiota reshaping around weaning and the increase of mainly Prevotella species just after weaning are equally important for resistance to postweaning diarrhea in piglets under field conditions.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40301 - Veterinary science

Projekt

<a href="/cs/project/QK1810462" target="_blank" >QK1810462: Manipulace se složením střevní mikroflóry monogastrů jako cesta k potlačení E. coli infekcí a snížení spotřeby antimikrobik</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Research in Veterinary Science

ISSN

0034-5288

e-ISSN

1532-2661

Svazek periodika

135

Číslo periodika v rámci svazku

MAR 2021

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

7

Strana od-do

59-65

Kód UT WoS článku

000632840800007

EID výsledku v databázi Scopus

2-s2.0-85099129112