Salmonella Typhimurium-based inactivated vaccine containing wide spectrum of bacterial antigens which mimics protein expression changes during different stages of an infection process

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F21%3AN0000058" target="_blank" >RIV/00027162:_____/21:N0000058 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Salmonella Typhimurium-based inactivated vaccine containing wide spectrum of bacterial antigens which mimics protein expression changes during different stages of an infection process

Popis výsledku v původním jazyce

Non-typhoid Salmonella infections constitute a persistent problem in veterinary medicine having considerable zoonotic potential. Among technical and hygienic measures, vaccination may be one of the options of how to prevent Salmonella infections in animal breedings and thus a transmission to human consumers. However, the efficiency of available commercial or experimental vaccines against non-typhoid Salmonella strains is not yet sufficient. We followed the path of inactivated vaccine that is safe and well-accepted but the antigen offer is limited. We improved this by diverse cultivation conditions mimicking bacterial protein expression during the natural infection process. The vaccine was prepared and applied to post-weaned piglets. A mixture of recombinant Salmonella proteins was also used as a recombinant vaccine for comparison. The efficiency of both approaches was determined by S. Typhimurium challenge. Animals vaccinated with the mixture of Salmonella cultures elicited lower increase in body temperature and lower bacterial burden of internal organs.

Název v anglickém jazyce

Salmonella Typhimurium-based inactivated vaccine containing wide spectrum of bacterial antigens which mimics protein expression changes during different stages of an infection process

Popis výsledku anglicky

Non-typhoid Salmonella infections constitute a persistent problem in veterinary medicine having considerable zoonotic potential. Among technical and hygienic measures, vaccination may be one of the options of how to prevent Salmonella infections in animal breedings and thus a transmission to human consumers. However, the efficiency of available commercial or experimental vaccines against non-typhoid Salmonella strains is not yet sufficient. We followed the path of inactivated vaccine that is safe and well-accepted but the antigen offer is limited. We improved this by diverse cultivation conditions mimicking bacterial protein expression during the natural infection process. The vaccine was prepared and applied to post-weaned piglets. A mixture of recombinant Salmonella proteins was also used as a recombinant vaccine for comparison. The efficiency of both approaches was determined by S. Typhimurium challenge. Animals vaccinated with the mixture of Salmonella cultures elicited lower increase in body temperature and lower bacterial burden of internal organs.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

<a href="/cs/project/TG03010038" target="_blank" >TG03010038: Rozvoj systému podpory "Proof-of-concept" na VÚVeL</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů