Healing and Angiogenic Properties of Collagen/Chitosan Scaffolds Enriched with Hyperstable FGF2-STAB® Protein: In Vitro, Ex Ovo and In Vivo Comprehensive Evaluation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F21%3AN0000067" target="_blank" >RIV/00027162:_____/21:N0000067 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.mdpi.com/2227-9059/9/6/590" target="_blank" >https://www.mdpi.com/2227-9059/9/6/590</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/biomedicines9060590" target="_blank" >10.3390/biomedicines9060590</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Healing and Angiogenic Properties of Collagen/Chitosan Scaffolds Enriched with Hyperstable FGF2-STAB® Protein: In Vitro, Ex Ovo and In Vivo Comprehensive Evaluation

Popis výsledku v původním jazyce

Wound healing is a process regulated by a complex interaction of multiple growth factors in-cluding fibroblast growth factor 2 (FGF2). Although FGF2 appears in several tissues engineered studies, its applications are limited due to its low stability both in vitro and in vivo. Here, this shortcoming is overcome by use a unique nine-point mutant of lowthe low molecular weight isoform FGF2 retaining full biological activity even after twenty days at 37 °C. Crosslinked freeze-dried 3D porous collagen-chitosan scaffolds enriched bywith this hyper stable recombi-nant human protein named as FGF2-STAB® are consequently tested in terms of their biocompat-ibility and cytotoxicity in vitro using murine 3T3-A31 fibroblasts, ex ovo on chick chorioallantoic membranes and in vivo on animal model of 3-month old White New Zealand rabbits. Metabolic activity assays indicated the positive effect of FGF2-STAB® already at very low concentrations (0.01 µg/mL). The angiogenic properties monitored ex ovo showed enhanced vascularization of the tested scaffolds. Histological evaluation and gene expression analysis by qRT-PCR proved newly formed granulation tissue at the place of a previous skin defect without significant in-flammation infiltration. This work highlights the safety and biocompatibility of newly devel-oped crosslinked collagen/chitosan scaffolds involving FGF2-STAB® protein. Moreover, these sponges could be used as scaffolds for growing cells in dermis replacement, where neovascular-ization is a crucial parameter for successful skin regeneration.

Název v anglickém jazyce

Healing and Angiogenic Properties of Collagen/Chitosan Scaffolds Enriched with Hyperstable FGF2-STAB® Protein: In Vitro, Ex Ovo and In Vivo Comprehensive Evaluation

Popis výsledku anglicky

Wound healing is a process regulated by a complex interaction of multiple growth factors in-cluding fibroblast growth factor 2 (FGF2). Although FGF2 appears in several tissues engineered studies, its applications are limited due to its low stability both in vitro and in vivo. Here, this shortcoming is overcome by use a unique nine-point mutant of lowthe low molecular weight isoform FGF2 retaining full biological activity even after twenty days at 37 °C. Crosslinked freeze-dried 3D porous collagen-chitosan scaffolds enriched bywith this hyper stable recombi-nant human protein named as FGF2-STAB® are consequently tested in terms of their biocompat-ibility and cytotoxicity in vitro using murine 3T3-A31 fibroblasts, ex ovo on chick chorioallantoic membranes and in vivo on animal model of 3-month old White New Zealand rabbits. Metabolic activity assays indicated the positive effect of FGF2-STAB® already at very low concentrations (0.01 µg/mL). The angiogenic properties monitored ex ovo showed enhanced vascularization of the tested scaffolds. Histological evaluation and gene expression analysis by qRT-PCR proved newly formed granulation tissue at the place of a previous skin defect without significant in-flammation infiltration. This work highlights the safety and biocompatibility of newly devel-oped crosslinked collagen/chitosan scaffolds involving FGF2-STAB® protein. Moreover, these sponges could be used as scaffolds for growing cells in dermis replacement, where neovascular-ization is a crucial parameter for successful skin regeneration.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40301 - Veterinary science

Projekt

<a href="/cs/project/NV17-29874A" target="_blank" >NV17-29874A: Optimalizace nanostrukturálních dermálních náhrad na zvířecím modelu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biomedicines

ISSN

2227-9059

e-ISSN

2227-9059

Svazek periodika

9

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

29

Strana od-do

—

Kód UT WoS článku

000665273200001

EID výsledku v databázi Scopus

2-s2.0-85107137204