Selected Carnivora species from the Czech Republic as a potential source of food-borne pathogens

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F21%3AN0000091" target="_blank" >RIV/00027162:_____/21:N0000091 - isvavai.cz</a>

Výsledek na webu

<a href="http://iwop2020.paru.cas.cz/conference-schedule/" target="_blank" >http://iwop2020.paru.cas.cz/conference-schedule/</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Selected Carnivora species from the Czech Republic as a potential source of food-borne pathogens

Popis výsledku v původním jazyce

Wildlife is the subject of a wide range of scientific studies, from ecological, through nature conservation to epidemiological. In our study, we examined representatives of selected species of small mammals from order Carnivora that can serve as a potential reservoir of pathogens capable of causing food-borne infections in humans. The study included faecal samples from 309 animals hunted in the years 2015 - 2020 in 11 regions of the Czech Republic. The examined animal species included 108 northern raccoons (Procyon lotor), 96 Eurasian badgers (Meles meles), 62 red foxes (Vulpes vulpes) and 43 raccoon dogs (Nyctereutes procyonoides). Samples were analysed for the presence of DNA of selected zoonotic bacteria and parasites (Campylobacter jejuni, Listeria monocytogenes, Salmonella enterica, Yersinia enterocolitica, Escherichia coli (serotype O26), Giardia spp., Cryptosporidium spp., Echinococcus multilocularis) using multiplex oligonucleotide ligation – polymerase chain reaction (MOL-PCR) with an adaptation to xMAP detection system. This provided a qualitative analysis of different nucleic acids from one biological sample simultaneously in one reaction. The most abundant pathogen in the faeces of wild animals was enterohemorrhagic E. coli O26, the least represented were bacteria Salmonella enterica and Listeria monocytogenes. Parasitic DNA was found only in the faeces of canine and E. multilocularis was detected only in samples from the red foxes. DNA of Cryptosporidium spp. was detected in the samples of ten animals (in five raccoon dogs and five red foxes), DNA of Giardia spp. only in faeces from six red foxes. Small mammals of the order Carnivora from the Czech Republic could be reservoir of selected food-borne pathogens and excrete them in faeces. Because the route of human infection is oral, situations that increase the risk of these infections should be avoided.

Název v anglickém jazyce

Selected Carnivora species from the Czech Republic as a potential source of food-borne pathogens

Popis výsledku anglicky

Wildlife is the subject of a wide range of scientific studies, from ecological, through nature conservation to epidemiological. In our study, we examined representatives of selected species of small mammals from order Carnivora that can serve as a potential reservoir of pathogens capable of causing food-borne infections in humans. The study included faecal samples from 309 animals hunted in the years 2015 - 2020 in 11 regions of the Czech Republic. The examined animal species included 108 northern raccoons (Procyon lotor), 96 Eurasian badgers (Meles meles), 62 red foxes (Vulpes vulpes) and 43 raccoon dogs (Nyctereutes procyonoides). Samples were analysed for the presence of DNA of selected zoonotic bacteria and parasites (Campylobacter jejuni, Listeria monocytogenes, Salmonella enterica, Yersinia enterocolitica, Escherichia coli (serotype O26), Giardia spp., Cryptosporidium spp., Echinococcus multilocularis) using multiplex oligonucleotide ligation – polymerase chain reaction (MOL-PCR) with an adaptation to xMAP detection system. This provided a qualitative analysis of different nucleic acids from one biological sample simultaneously in one reaction. The most abundant pathogen in the faeces of wild animals was enterohemorrhagic E. coli O26, the least represented were bacteria Salmonella enterica and Listeria monocytogenes. Parasitic DNA was found only in the faeces of canine and E. multilocularis was detected only in samples from the red foxes. DNA of Cryptosporidium spp. was detected in the samples of ten animals (in five raccoon dogs and five red foxes), DNA of Giardia spp. only in faeces from six red foxes. Small mammals of the order Carnivora from the Czech Republic could be reservoir of selected food-borne pathogens and excrete them in faeces. Because the route of human infection is oral, situations that increase the risk of these infections should be avoided.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů