Broad and potent neutralizing human antibodies to tick-borne flaviviruses protect mice from disease

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F21%3AN0000108" target="_blank" >RIV/00027162:_____/21:N0000108 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60077344:_____/21:00548797 RIV/62157124:16170/21:43879840

Výsledek na webu

<a href="https://rupress.org/jem/article/218/5/e20210236/211958/Broad-and-potent-neutralizing-human-antibodies-to" target="_blank" >https://rupress.org/jem/article/218/5/e20210236/211958/Broad-and-potent-neutralizing-human-antibodies-to</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1084/jem.20210236" target="_blank" >10.1084/jem.20210236</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Broad and potent neutralizing human antibodies to tick-borne flaviviruses protect mice from disease

Popis výsledku v původním jazyce

Tick-borne encephalitis virus (TBEV) is an emerging human pathogen that causes potentially fatal disease with no specific treatment. Mouse monoclonal antibodies are protective against TBEV, but little is known about the human antibody response to infection. Here, we report on the human neutralizing antibody response to TBEV in a cohort of infected and vaccinated individuals. Expanded clones of memory B cells expressed closely related anti-envelope domain III (EDIII) antibodies in both groups of volunteers. However, the most potent neutralizing antibodies, with IC50s below 1 ng/ml, were found only in individuals who recovered from natural infection. These antibodies also neutralized other tick-borne flaviviruses, including Langat, louping ill, Omsk hemorrhagic fever, Kyasanur forest disease, and Powassan viruses. Structural analysis revealed a conserved epitope near the lateral ridge of EDIII adjoining the EDI–EDIII hinge region. Prophylactic or early therapeutic antibody administration was effective at low doses in mice that were lethally infected with TBEV.

Název v anglickém jazyce

Broad and potent neutralizing human antibodies to tick-borne flaviviruses protect mice from disease

Popis výsledku anglicky

Tick-borne encephalitis virus (TBEV) is an emerging human pathogen that causes potentially fatal disease with no specific treatment. Mouse monoclonal antibodies are protective against TBEV, but little is known about the human antibody response to infection. Here, we report on the human neutralizing antibody response to TBEV in a cohort of infected and vaccinated individuals. Expanded clones of memory B cells expressed closely related anti-envelope domain III (EDIII) antibodies in both groups of volunteers. However, the most potent neutralizing antibodies, with IC50s below 1 ng/ml, were found only in individuals who recovered from natural infection. These antibodies also neutralized other tick-borne flaviviruses, including Langat, louping ill, Omsk hemorrhagic fever, Kyasanur forest disease, and Powassan viruses. Structural analysis revealed a conserved epitope near the lateral ridge of EDIII adjoining the EDI–EDIII hinge region. Prophylactic or early therapeutic antibody administration was effective at low doses in mice that were lethally infected with TBEV.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10607 - Virology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Experimental Medicine

ISSN

0022-1007

e-ISSN

1540-9538

Svazek periodika

218

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

22

Strana od-do

"e20210236"

Kód UT WoS článku

000655178400019

EID výsledku v databázi Scopus

2-s2.0-85104165502