Spiroplasma Isolated From Third-Generation Laboratory Colony Ixodes persulcatus Ticks

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F21%3AN0000110" target="_blank" >RIV/00027162:_____/21:N0000110 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60077344:_____/21:00552209 RIV/60460709:41340/21:85343

Výsledek na webu

<a href="https://www.frontiersin.org/articles/10.3389/fvets.2021.659786/full" target="_blank" >https://www.frontiersin.org/articles/10.3389/fvets.2021.659786/full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fvets.2021.659786" target="_blank" >10.3389/fvets.2021.659786</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Spiroplasma Isolated From Third-Generation Laboratory Colony Ixodes persulcatus Ticks

Popis výsledku v původním jazyce

Spiroplasma are vertically-transmitted endosymbionts of ticks and other arthropods. Field-collected Ixodes persulcatus have been reported to harbour Spiroplasma, but nothing is known about their persistence during laboratory colonisation of this tick species. We successfully isolated Spiroplasma from internal organs of 6/10 unfed adult ticks, belonging to the third generation of an I. persulcatus laboratory colony, into tick cell culture. We screened a further 51 adult male and female ticks from the same colony for presence of Spiroplasma by genus-specific PCR amplification of fragments of the 16S rRNA and rpoB genes; 100% of these ticks were infected and the 16S rRNA sequence showed 99.8% similarity to that of a previously-published Spiroplasma isolated from field-collected I. persulcatus. Our study shows that Spiroplasma endosymbionts persist at high prevalence in colonised I. persulcatus through at least three generations, and confirms the usefulness of tick cell lines for isolation and cultivation of this bacterium.

Název v anglickém jazyce

Spiroplasma Isolated From Third-Generation Laboratory Colony Ixodes persulcatus Ticks

Popis výsledku anglicky

Spiroplasma are vertically-transmitted endosymbionts of ticks and other arthropods. Field-collected Ixodes persulcatus have been reported to harbour Spiroplasma, but nothing is known about their persistence during laboratory colonisation of this tick species. We successfully isolated Spiroplasma from internal organs of 6/10 unfed adult ticks, belonging to the third generation of an I. persulcatus laboratory colony, into tick cell culture. We screened a further 51 adult male and female ticks from the same colony for presence of Spiroplasma by genus-specific PCR amplification of fragments of the 16S rRNA and rpoB genes; 100% of these ticks were infected and the 16S rRNA sequence showed 99.8% similarity to that of a previously-published Spiroplasma isolated from field-collected I. persulcatus. Our study shows that Spiroplasma endosymbionts persist at high prevalence in colonised I. persulcatus through at least three generations, and confirms the usefulness of tick cell lines for isolation and cultivation of this bacterium.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

FRONTIERS IN VETERINARY SCIENCE

ISSN

2297-1769

e-ISSN

2297-1769

Svazek periodika

8

Číslo periodika v rámci svazku

MAR 26 2021

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

10

Strana od-do

"659786"

Kód UT WoS článku

000637961700001

EID výsledku v databázi Scopus

2-s2.0-85103878204