RECENT CARP EDEMA VIRUS ISOLATES FROM CZECHIA ARE HIGHLY SIMILAR TO ISOLATES FROM CONTINENTAL EUROPE

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F21%3AN0000185" target="_blank" >RIV/00027162:_____/21:N0000185 - isvavai.cz</a>

Výsledek na webu

<a href="https://eposters.blob.core.windows.net/eas-eposters/AE2021AbstractBook.pdf" target="_blank" >https://eposters.blob.core.windows.net/eas-eposters/AE2021AbstractBook.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

RECENT CARP EDEMA VIRUS ISOLATES FROM CZECHIA ARE HIGHLY SIMILAR TO ISOLATES FROM CONTINENTAL EUROPE

Popis výsledku v původním jazyce

With a stable annual production of around 20 000 tonnes, common carp (Cyprinus carpio) is the most valuable aquaculture species in the Czech Republic. Emerging diseases, such as the carp edema virus disease (CEVD) detected in the country since 2013 (Matejickova et al. 2020) pose a threat to the industry. Molecular epidemiology, in the form of sequencing of isolates, can help understand the distribution of the virus in the country.Material and Methods: In total, 637 samples representing 124 locations (individual farms or ponds) were examined in the year 2020 for the presence of the virus using conventional and real-time PCR (Way et al, 2017). Partial P4a gene sequences (357 bp) of detected CEV isolates were obtained via Sanger sequencing and compared to isolated uploaded to the GenBank database. Results: CEV was diagnosed in six locations, namely four common carp farms and two koi carp ponds. Clinical signs in farmed common carp varied from asymptomatic to a 100% mortality rate in one of the facilities, meanwhile both positive koi in ponds showed sleepy-disease-like symptoms. Phylogenetically, two of the established CEV genogroups were detected: Genogroup I in common carp and genogroup IIa in koi. The isolates from common carp showed up to 100% identity with previously published sequences from Czechia, Germany, Hungary and Great Britain. Conclusions: High levels of similarity in the routinely sequenced partial P4a gene of carp edema virus suggest wide dissemination of the pathogen and possible transmission of specific isolates, most probably via the transport of live fish, ornamental or farmed.

Název v anglickém jazyce

RECENT CARP EDEMA VIRUS ISOLATES FROM CZECHIA ARE HIGHLY SIMILAR TO ISOLATES FROM CONTINENTAL EUROPE

Popis výsledku anglicky

With a stable annual production of around 20 000 tonnes, common carp (Cyprinus carpio) is the most valuable aquaculture species in the Czech Republic. Emerging diseases, such as the carp edema virus disease (CEVD) detected in the country since 2013 (Matejickova et al. 2020) pose a threat to the industry. Molecular epidemiology, in the form of sequencing of isolates, can help understand the distribution of the virus in the country.Material and Methods: In total, 637 samples representing 124 locations (individual farms or ponds) were examined in the year 2020 for the presence of the virus using conventional and real-time PCR (Way et al, 2017). Partial P4a gene sequences (357 bp) of detected CEV isolates were obtained via Sanger sequencing and compared to isolated uploaded to the GenBank database. Results: CEV was diagnosed in six locations, namely four common carp farms and two koi carp ponds. Clinical signs in farmed common carp varied from asymptomatic to a 100% mortality rate in one of the facilities, meanwhile both positive koi in ponds showed sleepy-disease-like symptoms. Phylogenetically, two of the established CEV genogroups were detected: Genogroup I in common carp and genogroup IIa in koi. The isolates from common carp showed up to 100% identity with previously published sequences from Czechia, Germany, Hungary and Great Britain. Conclusions: High levels of similarity in the routinely sequenced partial P4a gene of carp edema virus suggest wide dissemination of the pathogen and possible transmission of specific isolates, most probably via the transport of live fish, ornamental or farmed.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10607 - Virology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů