MOL-PCR and xMAP technology – a novel approach to the detection of African swine fever virus DNA

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F22%3AN0000032" target="_blank" >RIV/00027162:_____/22:N0000032 - isvavai.cz</a>

Výsledek na webu

<a href="https://actavet.vfu.cz/91/2/0141/" target="_blank" >https://actavet.vfu.cz/91/2/0141/</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2754/avb202291020141" target="_blank" >10.2754/avb202291020141</a>

Jazyk výsledku

angličtina

Název v původním jazyce

MOL-PCR and xMAP technology – a novel approach to the detection of African swine fever virus DNA

Popis výsledku v původním jazyce

African swine fever is an emerging infectious disease of the family Suidae causing serious problems in many countries. The causative agent, African swine fever virus, can transmitted by direct contact with infected animals or via a contaminated environment, fomites, feed, meat and products thereof. Rapid detection of the virus and early diagnosis of the disease could help in prevention of its spread between animals and through countries. Therefore, a multiple-analyte profile (xMAP) technology based on multiple oligonucleotide ligation (MOL) followed by polymerase chain reaction (MOL-PCR) was introduced and verified. A system targeting two independent loci of the virus genome was designed to increase the likelihood of different strains detection. An internal control was employed to verity the correct course of the analysis. The sensitivity was experimentally determined as 10 genomic copies of the virus in one l of isolated DNA. The system was verified on samples originating from a recent African swine fever outbreak in the Czech Republic (six spleen) and the Czech market (eight liver and heart tissues) with real-time polymerase chain reaction used as a reference method. The results of both methods were in agreement, even in samples with a low concentration of the virus genome (9.45 × 101 genomic copies/l of isolated DNA). The system introduced represents an open method allowing the detection and semi-quantification of up to 50 targets/agents in one reaction. It can, therefore, be used for rapid one-step screening and as an effective tool for risk management.

Název v anglickém jazyce

MOL-PCR and xMAP technology – a novel approach to the detection of African swine fever virus DNA

Popis výsledku anglicky

African swine fever is an emerging infectious disease of the family Suidae causing serious problems in many countries. The causative agent, African swine fever virus, can transmitted by direct contact with infected animals or via a contaminated environment, fomites, feed, meat and products thereof. Rapid detection of the virus and early diagnosis of the disease could help in prevention of its spread between animals and through countries. Therefore, a multiple-analyte profile (xMAP) technology based on multiple oligonucleotide ligation (MOL) followed by polymerase chain reaction (MOL-PCR) was introduced and verified. A system targeting two independent loci of the virus genome was designed to increase the likelihood of different strains detection. An internal control was employed to verity the correct course of the analysis. The sensitivity was experimentally determined as 10 genomic copies of the virus in one l of isolated DNA. The system was verified on samples originating from a recent African swine fever outbreak in the Czech Republic (six spleen) and the Czech market (eight liver and heart tissues) with real-time polymerase chain reaction used as a reference method. The results of both methods were in agreement, even in samples with a low concentration of the virus genome (9.45 × 101 genomic copies/l of isolated DNA). The system introduced represents an open method allowing the detection and semi-quantification of up to 50 targets/agents in one reaction. It can, therefore, be used for rapid one-step screening and as an effective tool for risk management.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10607 - Virology

Projekt

<a href="/cs/project/QK1920113" target="_blank" >QK1920113: Virus afrického moru prasat v mase a masných výrobcích – metody detekce a studium perzistence</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta Veterinaria Brno

ISSN

0001-7213

e-ISSN

1801-7576

Svazek periodika

91

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

9

Strana od-do

141-148

Kód UT WoS článku

000791588500004

EID výsledku v databázi Scopus

2-s2.0-85129472313