Hantavirus Brno loanvirus is highly specific to the common noctule bat (Nyctalus noctula) and widespread in Central Europe

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F23%3AN0000020" target="_blank" >RIV/00027162:_____/23:N0000020 - isvavai.cz</a>

Výsledek na webu

<a href="https://link.springer.com/article/10.1007/s11262-022-01952-2" target="_blank" >https://link.springer.com/article/10.1007/s11262-022-01952-2</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11262-022-01952-2" target="_blank" >10.1007/s11262-022-01952-2</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Hantavirus Brno loanvirus is highly specific to the common noctule bat (Nyctalus noctula) and widespread in Central Europe

Popis výsledku v původním jazyce

Bat-associated hantaviruses have been detected in Asia, Africa and Europe. Recently, a novel hantavirus (Brno loanvirus, BRNV) was identified in common noctule bats (Nyctalus noctula) in the Czech Republic, but nothing is known about its geographic range and prevalence. The objective of this study was to evaluate the distribution and host-specificity of BRNV by testing bats from neighbouring countries Germany, Austria and Poland. One thousand fourty-seven bats representing 21 species from Germany, 463 bats representing 18 species from Austria and 77 bats representing 12 species from Poland were screened by L segment broad-spectrum nested reverse transcription-polymerase chain reaction (RT-PCR) or by BRNV-specific realtime RT-PCR. Three common noctules from Germany, one common noctule from Austria and three common noctules from Poland were positive in the hantavirus RNA screening. Conventional RT-PCR and primer walking resulted in the amplification of partial L segment and (almost) complete S and M segment coding sequences for samples from Germany and partial L segment sequences for samples from Poland. Phylogenetic analysis of these nucleotide sequences showed highest similarity to BRNV from Czech Republic. The exclusive detection of BRNV in common noctules from different countries suggests high host specificity. The RNA detection rate in common noctules ranged between 0.5% (Austria), 1.2% (Germany) and 15% (Poland). In conclusion, this study demonstrates a broader distribution of BRNV in common noctules in Central Europe, but at low to moderate prevalence. Additional studies are needed to prove the zoonotic potential of this hantavirus and evaluate its transmission within ba populations.

Název v anglickém jazyce

Hantavirus Brno loanvirus is highly specific to the common noctule bat (Nyctalus noctula) and widespread in Central Europe

Popis výsledku anglicky

Bat-associated hantaviruses have been detected in Asia, Africa and Europe. Recently, a novel hantavirus (Brno loanvirus, BRNV) was identified in common noctule bats (Nyctalus noctula) in the Czech Republic, but nothing is known about its geographic range and prevalence. The objective of this study was to evaluate the distribution and host-specificity of BRNV by testing bats from neighbouring countries Germany, Austria and Poland. One thousand fourty-seven bats representing 21 species from Germany, 463 bats representing 18 species from Austria and 77 bats representing 12 species from Poland were screened by L segment broad-spectrum nested reverse transcription-polymerase chain reaction (RT-PCR) or by BRNV-specific realtime RT-PCR. Three common noctules from Germany, one common noctule from Austria and three common noctules from Poland were positive in the hantavirus RNA screening. Conventional RT-PCR and primer walking resulted in the amplification of partial L segment and (almost) complete S and M segment coding sequences for samples from Germany and partial L segment sequences for samples from Poland. Phylogenetic analysis of these nucleotide sequences showed highest similarity to BRNV from Czech Republic. The exclusive detection of BRNV in common noctules from different countries suggests high host specificity. The RNA detection rate in common noctules ranged between 0.5% (Austria), 1.2% (Germany) and 15% (Poland). In conclusion, this study demonstrates a broader distribution of BRNV in common noctules in Central Europe, but at low to moderate prevalence. Additional studies are needed to prove the zoonotic potential of this hantavirus and evaluate its transmission within ba populations.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10607 - Virology

Projekt

<a href="/cs/project/NU21-05-00143" target="_blank" >NU21-05-00143: Skryté zoonózy - odhalování nových patogenů z volné přírody</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Virus Genes

ISSN

0920-8569

e-ISSN

1572-994X

Svazek periodika

59

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

10

Strana od-do

323-332

Kód UT WoS článku

000901863200001

EID výsledku v databázi Scopus

2-s2.0-85144489037