Staphylococcus aureus Collagen-based Wound Dressings enriched with Thermostable Enzybiotics

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F23%3AN0000066" target="_blank" >RIV/00027162:_____/23:N0000066 - isvavai.cz</a>

Výsledek na webu

<a href="https://ubch.sci.muni.cz/pro-studenty/konference-doktorandu#abstracts" target="_blank" >https://ubch.sci.muni.cz/pro-studenty/konference-doktorandu#abstracts</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Staphylococcus aureus Collagen-based Wound Dressings enriched with Thermostable Enzybiotics

Popis výsledku v původním jazyce

Staphylococci, especially Staphylococcus aureus, are the most common cause of skin and soft tissue infections. Lytic enzymes originating from bacteria or bacteriophages represent alternative therapeutics for bacterial infection. The antibacterial effect results from their degrading activity towards bacterial peptidoglycan. Biopolymers, in addition to their function as a physical barrier and absorptive material, stimulate the wound healing process and can be used as a scaffold for therapeutics. We tested the antimicrobial activity of two recombinantly modified enzymes derived from lysostaphin (LYSSTAPH-S) and endolysin (LYSDERM-S). Thermostable mutations were introduced into the genetic information of each enzyme using seamless cloning. An increase in the thermal stability was verified by a thermal shift assay. Selected enzymes were incorporated in CMC/collagen or water-soluble foams. Antimicrobial susceptibility testing was performed by plate lysis and turbidity assay against MRSA strains. Our thermostable enzybiotics in wound dressing showed antimicrobial effect against MRSA pathogens and pointed out their potential for the treatment of staphylococcal wound infection.

Název v anglickém jazyce

Staphylococcus aureus Collagen-based Wound Dressings enriched with Thermostable Enzybiotics

Popis výsledku anglicky

Staphylococci, especially Staphylococcus aureus, are the most common cause of skin and soft tissue infections. Lytic enzymes originating from bacteria or bacteriophages represent alternative therapeutics for bacterial infection. The antibacterial effect results from their degrading activity towards bacterial peptidoglycan. Biopolymers, in addition to their function as a physical barrier and absorptive material, stimulate the wound healing process and can be used as a scaffold for therapeutics. We tested the antimicrobial activity of two recombinantly modified enzymes derived from lysostaphin (LYSSTAPH-S) and endolysin (LYSDERM-S). Thermostable mutations were introduced into the genetic information of each enzyme using seamless cloning. An increase in the thermal stability was verified by a thermal shift assay. Selected enzymes were incorporated in CMC/collagen or water-soluble foams. Antimicrobial susceptibility testing was performed by plate lysis and turbidity assay against MRSA strains. Our thermostable enzybiotics in wound dressing showed antimicrobial effect against MRSA pathogens and pointed out their potential for the treatment of staphylococcal wound infection.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/NU22-05-00475" target="_blank" >NU22-05-00475: Léčba infekcí způsobených multirezistentními kmeny bakterií pomocí nových antibakteriálních přípravků založených na katelicidinových antimikrobiálních peptidech</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů