Virulence of recent Czech IHNV and VHSV isolates assessed on three lines of rainbow trout

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F23%3AN0000078" target="_blank" >RIV/00027162:_____/23:N0000078 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.eurl-fish-crustacean.eu/fish/annual-workshop" target="_blank" >https://www.eurl-fish-crustacean.eu/fish/annual-workshop</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Virulence of recent Czech IHNV and VHSV isolates assessed on three lines of rainbow trout

Popis výsledku v původním jazyce

The presence of viral haemorrhagic septicaemia or infectious hematopoietic necrosis is sporadically detected in farmed rainbow trout in the Czech Republic, usually due to presence of patho-anatomical signs of the disease and increased mortality. In 2019, an IHNV strain with presumed low virulence was isolated from the ovarian fluid of a clinically healthy rainbow trout spawner. Virulence of the isolate was tested on rainbow trout fingerling (circa 3cm) during a 30-day infection trail. Two isolates of VHSV and an additional IHNV isolate from 2010 were administered in a bath challenge with a titre of 5,2x10^3 to 2,36x10^5. Survival of the fish varied from 0% (VSHV-2021) to 27% (IHNV-2010) and the results did not confirm the proposed low virulence of the IHNV2019 isolate, possibly due to the age difference between the trial fish and animal of isolate origin. Another trial was focused on the potential resistance of specific rainbow trout lines against IHNV and VHSV. Three defined trout lines were used – Denmark N30, Denmark N29 and Italy N15. The line DK-N30 was bred for a QTL for resistance against Flavobacterium infection. Immersion challenge of one IHNV and one VHSV isolate (titre 1x10^2 and 1x10^4, respectively) was performed. Mortalities, specific antibodies against the viruses, leucocyte counts, haematocrit, haemoglobin, phagocytic activity, and interferon activity were measured and compared, with only a few parameters showing statistical differences between the three rainbow trout lines.

Název v anglickém jazyce

Virulence of recent Czech IHNV and VHSV isolates assessed on three lines of rainbow trout

Popis výsledku anglicky

The presence of viral haemorrhagic septicaemia or infectious hematopoietic necrosis is sporadically detected in farmed rainbow trout in the Czech Republic, usually due to presence of patho-anatomical signs of the disease and increased mortality. In 2019, an IHNV strain with presumed low virulence was isolated from the ovarian fluid of a clinically healthy rainbow trout spawner. Virulence of the isolate was tested on rainbow trout fingerling (circa 3cm) during a 30-day infection trail. Two isolates of VHSV and an additional IHNV isolate from 2010 were administered in a bath challenge with a titre of 5,2x10^3 to 2,36x10^5. Survival of the fish varied from 0% (VSHV-2021) to 27% (IHNV-2010) and the results did not confirm the proposed low virulence of the IHNV2019 isolate, possibly due to the age difference between the trial fish and animal of isolate origin. Another trial was focused on the potential resistance of specific rainbow trout lines against IHNV and VHSV. Three defined trout lines were used – Denmark N30, Denmark N29 and Italy N15. The line DK-N30 was bred for a QTL for resistance against Flavobacterium infection. Immersion challenge of one IHNV and one VHSV isolate (titre 1x10^2 and 1x10^4, respectively) was performed. Mortalities, specific antibodies against the viruses, leucocyte counts, haematocrit, haemoglobin, phagocytic activity, and interferon activity were measured and compared, with only a few parameters showing statistical differences between the three rainbow trout lines.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40103 - Fishery

Projekt

<a href="/cs/project/EF16_019%2F0000869" target="_blank" >EF16_019/0000869: Udržitelná produkce zdravých ryb v různých akvakulturních systémech - PROFISH</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů