Can the examination of different types of hive samples be a non-invasive method for detection and quantification of viruses in honey bee (Apis mellifera L.) colonies?

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F23%3AN0000140" target="_blank" >RIV/00027162:_____/23:N0000140 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62157124:16270/23:43880852

Výsledek na webu

<a href="https://sciendo.com/article/10.2478/jvetres-2023-0046" target="_blank" >https://sciendo.com/article/10.2478/jvetres-2023-0046</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2478/jvetres-2023-0046" target="_blank" >10.2478/jvetres-2023-0046</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Can the examination of different types of hive samples be a non-invasive method for detection and quantification of viruses in honey bee (Apis mellifera L.) colonies?

Popis výsledku v původním jazyce

Introduction: Honey bee viruses have been shown to negatively affect the vigour and longevity of European honey bees (Apismellifera L). In the present work, beehive materials were tested for their potential to serve as non-invasive samples for honey beevirus detection. Material and Methods: Honey, pollen, hive debris, hive grid smears and forager honey bees were collectedfrom 24 hives at four locations in the Czech Republic. Deformed wing virus (DWV), acute bee paralysis virus (ABPV), sacbroodvirus (SBV) and black queen cell virus (BQCV) were detected using a reverse transcription PCR (RT-PCR) and real-timequantitative RT-PCR and the results for bees and alternative materials compared. Results: All forager bee samples containedDWV, BQCV and SBV and 54.2% had ABPV. When comparing beehive materials to bees, the most promising results wereobtained from honey and pollen samples, with BQCV and SBV detected in all honey samples and ABPV in 12.5%. Detection ofSBV was achieved in 91.6% of pollen samples, detection of BQCV in 87.5% and detection of DWW in 75%. The results fordebris and smears were less consistent with the viral profile of the forager samples. Conclusion: The best candidate materials forhoney bee virus detection in a non-invasive technique are honey and pollen.

Název v anglickém jazyce

Can the examination of different types of hive samples be a non-invasive method for detection and quantification of viruses in honey bee (Apis mellifera L.) colonies?

Popis výsledku anglicky

Introduction: Honey bee viruses have been shown to negatively affect the vigour and longevity of European honey bees (Apismellifera L). In the present work, beehive materials were tested for their potential to serve as non-invasive samples for honey beevirus detection. Material and Methods: Honey, pollen, hive debris, hive grid smears and forager honey bees were collectedfrom 24 hives at four locations in the Czech Republic. Deformed wing virus (DWV), acute bee paralysis virus (ABPV), sacbroodvirus (SBV) and black queen cell virus (BQCV) were detected using a reverse transcription PCR (RT-PCR) and real-timequantitative RT-PCR and the results for bees and alternative materials compared. Results: All forager bee samples containedDWV, BQCV and SBV and 54.2% had ABPV. When comparing beehive materials to bees, the most promising results wereobtained from honey and pollen samples, with BQCV and SBV detected in all honey samples and ABPV in 12.5%. Detection ofSBV was achieved in 91.6% of pollen samples, detection of BQCV in 87.5% and detection of DWW in 75%. The results fordebris and smears were less consistent with the viral profile of the forager samples. Conclusion: The best candidate materials forhoney bee virus detection in a non-invasive technique are honey and pollen.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10607 - Virology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Veterinary Research

ISSN

2450-7393

e-ISSN

2450-8608

Svazek periodika

67

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

9

Strana od-do

323-331

Kód UT WoS článku

001065223400001

EID výsledku v databázi Scopus

2-s2.0-85173547728