UV-C irradiation as an effective tool for sterilization of porcine chimeric VP1-PCV2bCap recombinant vaccine

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F23%3AN0000166" target="_blank" >RIV/00027162:_____/23:N0000166 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/23:10477443

Výsledek na webu

<a href="https://www.nature.com/articles/s41598-023-46791-9" target="_blank" >https://www.nature.com/articles/s41598-023-46791-9</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41598-023-46791-9" target="_blank" >10.1038/s41598-023-46791-9</a>

Jazyk výsledku

angličtina

Název v původním jazyce

UV-C irradiation as an effective tool for sterilization of porcine chimeric VP1-PCV2bCap recombinant vaccine

Popis výsledku v původním jazyce

Ultraviolet irradiation is an efective method of virus and bacteria inactivation. The dose of UV-C light necessary for baculovirus inactivation by measurement of fuorescent GFP protein produced by baculovirus expression system after the irradiation of baculovirus culture in doses ranging from 3.5 to 42 J/m2 was determined. At a dose of 36.8 J/m2 , only 0.5% of GFP-expressing cells were detected by fow cytometry and confocal microscopy. The stability of purifed VP1-PCV2bCap protein produced by baculovirus expression system was analyzed after the irradiation at doses ranging from 3.5 to 19.3 J/m2 . Up to the dose of 11 J/m2 , no signifcant efect of UV-C light on the stability of VP1PCV2bCap was detected. We observed a dose-dependent increase in VP1-PCV2bCap-specifc immune response in BALB/c mice immunized by recombinant protein sterilized by irradiation in dose 11 J/m2 with no signifcant diference between vaccines sterilized by UV-C light and fltration. A substantial diference in the production of VP1-PCV2bCap specifc IgG was observed in piglets immunized with VP1-PCV2bCap sterilized by UV-C in comparison with protein sterilized by fltration in combination with the inactivation of baculovirus by binary ethylenimine. UV-C irradiation represents an efective method for vaccine sterilization, where commonly used methods of sterilization are not possible.

Název v anglickém jazyce

UV-C irradiation as an effective tool for sterilization of porcine chimeric VP1-PCV2bCap recombinant vaccine

Popis výsledku anglicky

Ultraviolet irradiation is an efective method of virus and bacteria inactivation. The dose of UV-C light necessary for baculovirus inactivation by measurement of fuorescent GFP protein produced by baculovirus expression system after the irradiation of baculovirus culture in doses ranging from 3.5 to 42 J/m2 was determined. At a dose of 36.8 J/m2 , only 0.5% of GFP-expressing cells were detected by fow cytometry and confocal microscopy. The stability of purifed VP1-PCV2bCap protein produced by baculovirus expression system was analyzed after the irradiation at doses ranging from 3.5 to 19.3 J/m2 . Up to the dose of 11 J/m2 , no signifcant efect of UV-C light on the stability of VP1PCV2bCap was detected. We observed a dose-dependent increase in VP1-PCV2bCap-specifc immune response in BALB/c mice immunized by recombinant protein sterilized by irradiation in dose 11 J/m2 with no signifcant diference between vaccines sterilized by UV-C light and fltration. A substantial diference in the production of VP1-PCV2bCap specifc IgG was observed in piglets immunized with VP1-PCV2bCap sterilized by UV-C in comparison with protein sterilized by fltration in combination with the inactivation of baculovirus by binary ethylenimine. UV-C irradiation represents an efective method for vaccine sterilization, where commonly used methods of sterilization are not possible.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40301 - Veterinary science

Projekt

<a href="/cs/project/EF16_025%2F0007397" target="_blank" >EF16_025/0007397: Centrum pro Rekombinantní biotechnologie a imunoterapeutika</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Scientific Reports

ISSN

2045-2322

e-ISSN

2045-2322

Svazek periodika

13

Číslo periodika v rámci svazku

2023

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

11

Strana od-do

—

Kód UT WoS článku

001101369900095

EID výsledku v databázi Scopus

2-s2.0-85175966726