Utilization of the Actiphage®-IS900 qPCR assay as a diagnostic tool for rapid determination of paratuberculosis infection status in small ruminant herds

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F23%3AN0000180" target="_blank" >RIV/00027162:_____/23:N0000180 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Utilization of the Actiphage®-IS900 qPCR assay as a diagnostic tool for rapid determination of paratuberculosis infection status in small ruminant herds

Popis výsledku v původním jazyce

Introduction: Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of paratuberculosis, a chronic infectious intestinal disease occurring in domestic and wild ruminants. Early diagnosis of infected herds enabling timely adoption of control measures is tremendously important in view of the fact that the disease has a significant economic impact on farmers. The aim of this study was to evaluate the possibility of rapid detection of viable MAP on small ruminant farms based on environmental sample examination using a novel phage-based approach named Actiphage®. Material and methods: A total of 9 fresh and 28 frozen (8 or 11 years at -70 °C) environmental samples originating from paratuberculosis-affected farms were analysed by 4 different diagnostic methods: Actiphage-IS900 real-time PCR (qPCR), conventional phage amplification assay, culture and direct IS900 qPCR. Results: Viable MAP was detected in 1 fresh environmental sample using Actiphage-IS900 qPCR. None of the frozen samples tested positive using Actiphage-IS900 qPCR, which was consistent with the results of culture examination also providing information on viability. Conclusion: This study innovatively describes other possible uses of phage-based methods in paratuberculosis control strategies. Actiphage-qPCR was found to be less laborious compared to culture and provided results within 6 hours, suggesting that Actiphage-qPCR may be a valuable tool for rapid initial determination of the infectious status of farmed animals based on environmental sample examination. Acknowledgements: This work was supported by grants from the Ministry of Agriculture of the Czech Republic QK1910082 and RVO0518.

Název v anglickém jazyce

Utilization of the Actiphage®-IS900 qPCR assay as a diagnostic tool for rapid determination of paratuberculosis infection status in small ruminant herds

Popis výsledku anglicky

Introduction: Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of paratuberculosis, a chronic infectious intestinal disease occurring in domestic and wild ruminants. Early diagnosis of infected herds enabling timely adoption of control measures is tremendously important in view of the fact that the disease has a significant economic impact on farmers. The aim of this study was to evaluate the possibility of rapid detection of viable MAP on small ruminant farms based on environmental sample examination using a novel phage-based approach named Actiphage®. Material and methods: A total of 9 fresh and 28 frozen (8 or 11 years at -70 °C) environmental samples originating from paratuberculosis-affected farms were analysed by 4 different diagnostic methods: Actiphage-IS900 real-time PCR (qPCR), conventional phage amplification assay, culture and direct IS900 qPCR. Results: Viable MAP was detected in 1 fresh environmental sample using Actiphage-IS900 qPCR. None of the frozen samples tested positive using Actiphage-IS900 qPCR, which was consistent with the results of culture examination also providing information on viability. Conclusion: This study innovatively describes other possible uses of phage-based methods in paratuberculosis control strategies. Actiphage-qPCR was found to be less laborious compared to culture and provided results within 6 hours, suggesting that Actiphage-qPCR may be a valuable tool for rapid initial determination of the infectious status of farmed animals based on environmental sample examination. Acknowledgements: This work was supported by grants from the Ministry of Agriculture of the Czech Republic QK1910082 and RVO0518.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

<a href="/cs/project/QK1910082" target="_blank" >QK1910082: Řešení problematiky výskytu bakteriálních, protozoárních a virových zoonotických agens v chovech malých přežvýkavců</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů