Prevalence of proliferative kidney disease in the rivers of the Czech Republic

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F24%3AN0000217" target="_blank" >RIV/00027162:_____/24:N0000217 - isvavai.cz</a>

Výsledek na webu

<a href="https://eafp.org/the-1st-meeting-of-the-central-and-eastern-european-eafp-branch-2nd-announcement/" target="_blank" >https://eafp.org/the-1st-meeting-of-the-central-and-eastern-european-eafp-branch-2nd-announcement/</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Prevalence of proliferative kidney disease in the rivers of the Czech Republic

Popis výsledku v původním jazyce

Novotná, H., Palíková, M., Motlová, J., Mikulíková, I., Papežíková, I., Toulová, I., Pojezdal, Ľ., 2024. Prevalence of proliferative kidney disease in the rivers of the Czech Republic. The 1st Meeting of the Central and Eastern European EAFP Branches, Abstract Book, European Association of Fish Pathologists, Křtiny, Czech Republic, 4.–6. 12. 2024, p. 13. ISBN 978-80-7701-005-4. Proliferative kidney disease (PKD) affects both wild and farmed salmonid fish, causes losses in aquaculture and a substantial decline in brown trout populations throughout Europe. The causative agent, Tetracapsuloides bryosalmonae, is an endoparasite (Myxozoa: Malacosporea) and its life cycle involves freshwater bryozoans as invertebrate hosts. The kidney represents the main target organ in fish. In the lumen of the kidney tubules, T. bryosalmonae forms sporogonic developmental stages and can be detected in this organ with the use of molecular methods. With climate change, more severe PKD cases are to be anticipated, as the parasite is highly temperature-dependent. The aim of this study was to evaluate the prevalence of T. bryosalmonae in wild brown trout (Salmo trutta) in the rivers of the Czech Republic. Rivers of three basins (Elbe, Morava and Oder) were investigated, and in total, fish from 62 locations from 42 unique streams were sampled. Where possible, at least ten fish per location were caught by electrofishing. The animals were euthanised, measurements were taken and the pathoanatomical and parasitological examinations (including collection and taxonomic determination of parasites from the skin, fins and gills by light microscopy) were conducted on-site. Tissue samples from each individual were collected and stored in 70% ethanol for parasitological examination (caudal kidney) and virological examination (haematopoietic organs: cranial kidney, spleen and heart) using molecular methods. Ethanol-stored samples of the caudal kidney were examined by real-time PCR for the presence of T. bryosalmonae. The fish were also examined for the presence of salmonid viruses (VHS, IHN, PRV-3, and IPN). Swelling of the caudal kidney, indicative of the presence of T. bryosalmonae, was the most common pathoanatomical sign present in the fish, as confirmed by the PCR results. High prevalence of the parasite in wild brown trout in the Czech Republic was confirmed."

Název v anglickém jazyce

Prevalence of proliferative kidney disease in the rivers of the Czech Republic

Popis výsledku anglicky

Novotná, H., Palíková, M., Motlová, J., Mikulíková, I., Papežíková, I., Toulová, I., Pojezdal, Ľ., 2024. Prevalence of proliferative kidney disease in the rivers of the Czech Republic. The 1st Meeting of the Central and Eastern European EAFP Branches, Abstract Book, European Association of Fish Pathologists, Křtiny, Czech Republic, 4.–6. 12. 2024, p. 13. ISBN 978-80-7701-005-4. Proliferative kidney disease (PKD) affects both wild and farmed salmonid fish, causes losses in aquaculture and a substantial decline in brown trout populations throughout Europe. The causative agent, Tetracapsuloides bryosalmonae, is an endoparasite (Myxozoa: Malacosporea) and its life cycle involves freshwater bryozoans as invertebrate hosts. The kidney represents the main target organ in fish. In the lumen of the kidney tubules, T. bryosalmonae forms sporogonic developmental stages and can be detected in this organ with the use of molecular methods. With climate change, more severe PKD cases are to be anticipated, as the parasite is highly temperature-dependent. The aim of this study was to evaluate the prevalence of T. bryosalmonae in wild brown trout (Salmo trutta) in the rivers of the Czech Republic. Rivers of three basins (Elbe, Morava and Oder) were investigated, and in total, fish from 62 locations from 42 unique streams were sampled. Where possible, at least ten fish per location were caught by electrofishing. The animals were euthanised, measurements were taken and the pathoanatomical and parasitological examinations (including collection and taxonomic determination of parasites from the skin, fins and gills by light microscopy) were conducted on-site. Tissue samples from each individual were collected and stored in 70% ethanol for parasitological examination (caudal kidney) and virological examination (haematopoietic organs: cranial kidney, spleen and heart) using molecular methods. Ethanol-stored samples of the caudal kidney were examined by real-time PCR for the presence of T. bryosalmonae. The fish were also examined for the presence of salmonid viruses (VHS, IHN, PRV-3, and IPN). Swelling of the caudal kidney, indicative of the presence of T. bryosalmonae, was the most common pathoanatomical sign present in the fish, as confirmed by the PCR results. High prevalence of the parasite in wild brown trout in the Czech Republic was confirmed."

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů