Caviplasma technology as a tool for management of ichthyophthiriasis

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Caviplasma technology as a tool for management of ichthyophthiriasis

Popis výsledku v původním jazyce

Papežíková, I., Toulová, I., Palíková, M., Mikulíková, I., Novotná, H., Mendel, J., 2024. Caviplasma technology as a tool for management of ichthyophthiriasis. The 1st Meeting of the Central and Eastern European EAFP Branches, Abstract Book, European Association of Fish Pathologists, Křtiny, Czech Republic, 4.–6. 12. 2024, p. 17. ISBN 978-80-7701-005-4. lchthyophthirius multifiliis is a parasitic ciliate causing one of the most serious fish diseases, white spot disease, which is associated with high morbidity, mortality and significant economic losses in the aquaculture. In the first part of the presented work, we tested the novel technology called CaviPlasma for treatment of water contaminated with free-swimming stages of I. multifiliis. Subsequently, we tested the treatment of water supplying recirculation tanks with fish suffering from ichthyophthiriasis. CaviPlasma technology is based on the synergetic effect of hydrodynamic cavitation in a fast-flowing liquid and electric discharges generated within the water vapour inside the cavitation bubbles. This leads to large-volume generation of UV radiation and reactive oxygen species with biocidal effect. Experiment 1. Experiment was performed on healthy juvenile wells catfish (Silurus glanis) with a weight of 6,3 ± 1,3 g and total length of 102,7 ± 8,9 mm (n =8). Fish were divided into four experimental groups and let to acclimatize for one week. After that, sample of tap water containing free stages of I. multifiliis was treated with CaviPlasma (Batch 1 for 80 s and Batch 2 for 420 s). Batch 3 was left untreated. Batch 4 was untreated tap water that did not contain I. multifiliis. Two hours later, four above mentioned batches of water were added into tanks with four experimental groups of fish. Sampling of fish was performed on the 6th day after the start of the experiment. Fish were euthanized, skin and gill smears were performed and numbers of I. multifiliis were detected microscopically. In fish from the group which obtained untreated water with I. multifilis, massive ichthyophthiriasis was detected in both skin and gills. No parasites were found in any other group. Experiment 2. Treatment of inflow water with CaviPlasma was tested in recirculation tanks with adult common carp (Cyprinus carpio) with ongoing ichthyophthiriasis. Different modes of water treatment were tested; the length and frequency of the treatment was adjusted according to the concentration of reactive oxygen species detected in the treated water. Experiment lasted for three weeks. None of the treatment modes led to cure or suppression of ongoing infection in the diseased fish. Conclusion: Experiment I shows that CaviPlasma was able to devitalize free-living stages of I. multifiliis completely and could be therefore a very effective tool for preventive treatment of inflow water. On the other hand, water treatment was ineffective in the case of ongoing ichthyophthiriasis (Experiment 2).

Název v anglickém jazyce

Caviplasma technology as a tool for management of ichthyophthiriasis

Popis výsledku anglicky

Papežíková, I., Toulová, I., Palíková, M., Mikulíková, I., Novotná, H., Mendel, J., 2024. Caviplasma technology as a tool for management of ichthyophthiriasis. The 1st Meeting of the Central and Eastern European EAFP Branches, Abstract Book, European Association of Fish Pathologists, Křtiny, Czech Republic, 4.–6. 12. 2024, p. 17. ISBN 978-80-7701-005-4. lchthyophthirius multifiliis is a parasitic ciliate causing one of the most serious fish diseases, white spot disease, which is associated with high morbidity, mortality and significant economic losses in the aquaculture. In the first part of the presented work, we tested the novel technology called CaviPlasma for treatment of water contaminated with free-swimming stages of I. multifiliis. Subsequently, we tested the treatment of water supplying recirculation tanks with fish suffering from ichthyophthiriasis. CaviPlasma technology is based on the synergetic effect of hydrodynamic cavitation in a fast-flowing liquid and electric discharges generated within the water vapour inside the cavitation bubbles. This leads to large-volume generation of UV radiation and reactive oxygen species with biocidal effect. Experiment 1. Experiment was performed on healthy juvenile wells catfish (Silurus glanis) with a weight of 6,3 ± 1,3 g and total length of 102,7 ± 8,9 mm (n =8). Fish were divided into four experimental groups and let to acclimatize for one week. After that, sample of tap water containing free stages of I. multifiliis was treated with CaviPlasma (Batch 1 for 80 s and Batch 2 for 420 s). Batch 3 was left untreated. Batch 4 was untreated tap water that did not contain I. multifiliis. Two hours later, four above mentioned batches of water were added into tanks with four experimental groups of fish. Sampling of fish was performed on the 6th day after the start of the experiment. Fish were euthanized, skin and gill smears were performed and numbers of I. multifiliis were detected microscopically. In fish from the group which obtained untreated water with I. multifilis, massive ichthyophthiriasis was detected in both skin and gills. No parasites were found in any other group. Experiment 2. Treatment of inflow water with CaviPlasma was tested in recirculation tanks with adult common carp (Cyprinus carpio) with ongoing ichthyophthiriasis. Different modes of water treatment were tested; the length and frequency of the treatment was adjusted according to the concentration of reactive oxygen species detected in the treated water. Experiment lasted for three weeks. None of the treatment modes led to cure or suppression of ongoing infection in the diseased fish. Conclusion: Experiment I shows that CaviPlasma was able to devitalize free-living stages of I. multifiliis completely and could be therefore a very effective tool for preventive treatment of inflow water. On the other hand, water treatment was ineffective in the case of ongoing ichthyophthiriasis (Experiment 2).

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů