Extraintestinal Escherichia coli from camel carcasses: Phylogroups, serotypes, and markers of virulence

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F25%3AN0000096" target="_blank" >RIV/00027162:_____/25:N0000096 - isvavai.cz</a>

Výsledek na webu

<a href="https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0334045" target="_blank" >https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0334045</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1371/journal.pone.0334045" target="_blank" >10.1371/journal.pone.0334045</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Extraintestinal Escherichia coli from camel carcasses: Phylogroups, serotypes, and markers of virulence

Popis výsledku v původním jazyce

Pathogenic Escherichia coli causes infections responsible for economic losses in animal herds worldwide. Although this bacterium is well studied in livestock and poultry, studies of camelid infections caused by extraintestinal pathogenic E. coli (ExPEC) are limited. In this study, a set of ExPEC from camel carcasses (n = 150) was characterized with respect to phylogenetic groups, 162 O serotypes, and 35 virulence-associated genes (VAGs) using PCR screening. ExPEC frequently belonged to phylogroup B1 (58.7%), followed by phylogroups C, A, and B2 (12.7%, 12.0%, and 9.3%, respectively). Additionally, the set of ExPEC contained 36 different serotypes. The ExPEC isolates were found to typically encode ≥5 tested VAGs, particularly those related to adhesion (afaI, fimA, pap, sfa, tsh), iron acquisition (fyuA, iroN, iucC, sitA), host cell damage (α-hly, cdt, cnf1, sat), invasion (ibeA), and bacterial protection (iss, ompT, traT). Moreover, ExPEC from camel adults and calves were different from each other. Among isolates from calves, prevalence was significantly higher for phylogroups C (q < 0.001) and E (q < 0.01), and ten VAGs, including fitness factors (eitA, fepC, fyuA, iroN, iss, iucD, ompT, sitA), as well as VAGs with stronger link to pathogenicity (hlyF, and pap). To identify potential reservoir of camel ExPEC strains, fecal E. coli (n = 139) from healthy camels were also analyzed. Based on the identified characteristics, ExPEC were distinguishable from fecal isolates of healthy camels, suggesting an exogenous source of ExPEC infections, likely transmitted from wild birds and human keepers.

Název v anglickém jazyce

Extraintestinal Escherichia coli from camel carcasses: Phylogroups, serotypes, and markers of virulence

Popis výsledku anglicky

Pathogenic Escherichia coli causes infections responsible for economic losses in animal herds worldwide. Although this bacterium is well studied in livestock and poultry, studies of camelid infections caused by extraintestinal pathogenic E. coli (ExPEC) are limited. In this study, a set of ExPEC from camel carcasses (n = 150) was characterized with respect to phylogenetic groups, 162 O serotypes, and 35 virulence-associated genes (VAGs) using PCR screening. ExPEC frequently belonged to phylogroup B1 (58.7%), followed by phylogroups C, A, and B2 (12.7%, 12.0%, and 9.3%, respectively). Additionally, the set of ExPEC contained 36 different serotypes. The ExPEC isolates were found to typically encode ≥5 tested VAGs, particularly those related to adhesion (afaI, fimA, pap, sfa, tsh), iron acquisition (fyuA, iroN, iucC, sitA), host cell damage (α-hly, cdt, cnf1, sat), invasion (ibeA), and bacterial protection (iss, ompT, traT). Moreover, ExPEC from camel adults and calves were different from each other. Among isolates from calves, prevalence was significantly higher for phylogroups C (q < 0.001) and E (q < 0.01), and ten VAGs, including fitness factors (eitA, fepC, fyuA, iroN, iss, iucD, ompT, sitA), as well as VAGs with stronger link to pathogenicity (hlyF, and pap). To identify potential reservoir of camel ExPEC strains, fecal E. coli (n = 139) from healthy camels were also analyzed. Based on the identified characteristics, ExPEC were distinguishable from fecal isolates of healthy camels, suggesting an exogenous source of ExPEC infections, likely transmitted from wild birds and human keepers.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40301 - Veterinary science

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plos One

ISSN

—

e-ISSN

1932-6203

Svazek periodika

20

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

14

Strana od-do

—

Kód UT WoS článku

001591276000012

EID výsledku v databázi Scopus

2-s2.0-105018273126