Onion thiolanes: multifunctional modulators of oxidative stress, PPAR-γ2 signaling, and bacterial growth

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F25%3AN0000199" target="_blank" >RIV/00027162:_____/25:N0000199 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Onion thiolanes: multifunctional modulators of oxidative stress, PPAR-γ2 signaling, and bacterial growth

Popis výsledku v původním jazyce

Phytochemical Society of Europe Trends in Natural Product Research 2025 Young Scientists Meeting, Wroclaw, Polsko 2 - 6 June 2025 – poster. Onions (Allium cepa L.) are known for their sulfur-containing compounds with reported biological activities. In this study, 24 thiolane derivatives isolated from onions were evaluated for their potential effects on oxidative stress, metabolic regulation, NF-κB signaling, and antimicrobial properties. At a concentration of 10 µM, none of the compounds markedly impaired THP1-Blue™ NF-κB cell viability; however, several compounds demonstrated moderate cytotoxicity at higher concentrations (50 µM). Given the previously reported anti-inflammatory potential of sulfur-containing compounds, a reduction in NF-κB pathway activity was anticipated [1]. Most thiolanes showed minimal or no inhibition of LPS-induced NF-κB activation, indicating alternative anti-inflammatory mechanisms. Most reduced ROS levels under oxidative stress, though cepadithiolactones A1–A3 were mildly prooxidative. Selected thiolanes (A5–A7, B4, B5) activated the PPAR-γ2 pathway, achieving up to 26% of the activity induced by rosiglitazone. Thiolanes from onion exhibited limited antimicrobial activity against E. coli and C. albicans at the tested concentrations. However, certain thiolanes showed inhibitory effects against S. aureus subsp. aureus, with minimum inhibitory concentrations ranging from 3.125 to 6.25 mM. These findings suggest that onion-derived thiolanes possess moderate antioxidant capacity and metabolic modulatory properties, with selective antimicrobial effects and limited anti-inflammatory activity via NF-κB inhibition. Further studies are needed to elucidate alternative anti-inflammatory mechanisms and explore their potential applications in functional food development.

Název v anglickém jazyce

Onion thiolanes: multifunctional modulators of oxidative stress, PPAR-γ2 signaling, and bacterial growth

Popis výsledku anglicky

Phytochemical Society of Europe Trends in Natural Product Research 2025 Young Scientists Meeting, Wroclaw, Polsko 2 - 6 June 2025 – poster. Onions (Allium cepa L.) are known for their sulfur-containing compounds with reported biological activities. In this study, 24 thiolane derivatives isolated from onions were evaluated for their potential effects on oxidative stress, metabolic regulation, NF-κB signaling, and antimicrobial properties. At a concentration of 10 µM, none of the compounds markedly impaired THP1-Blue™ NF-κB cell viability; however, several compounds demonstrated moderate cytotoxicity at higher concentrations (50 µM). Given the previously reported anti-inflammatory potential of sulfur-containing compounds, a reduction in NF-κB pathway activity was anticipated [1]. Most thiolanes showed minimal or no inhibition of LPS-induced NF-κB activation, indicating alternative anti-inflammatory mechanisms. Most reduced ROS levels under oxidative stress, though cepadithiolactones A1–A3 were mildly prooxidative. Selected thiolanes (A5–A7, B4, B5) activated the PPAR-γ2 pathway, achieving up to 26% of the activity induced by rosiglitazone. Thiolanes from onion exhibited limited antimicrobial activity against E. coli and C. albicans at the tested concentrations. However, certain thiolanes showed inhibitory effects against S. aureus subsp. aureus, with minimum inhibitory concentrations ranging from 3.125 to 6.25 mM. These findings suggest that onion-derived thiolanes possess moderate antioxidant capacity and metabolic modulatory properties, with selective antimicrobial effects and limited anti-inflammatory activity via NF-κB inhibition. Further studies are needed to elucidate alternative anti-inflammatory mechanisms and explore their potential applications in functional food development.

Druh

O - Ostatní výsledky

CEP obor

—

OECD FORD obor

30104 - Pharmacology and pharmacy

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů