Identification of miRNAs with diagnostic potential in extracellular vesicles circulating in cerebrospinal fluid of glioma patients

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F25%3AN0000207" target="_blank" >RIV/00027162:_____/25:N0000207 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00027162:_____/25:N0000206

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Identification of miRNAs with diagnostic potential in extracellular vesicles circulating in cerebrospinal fluid of glioma patients

Popis výsledku v původním jazyce

CzeSEV meeting on Extracellular Vesicles 2025 (ISBN of Proceedings: 978-80-11-07777-8), Vestec, 11.11.2025, poster. Gliomas are highly heterogeneous tumors characterized by diverse molecular and histological profiles, resulting in variable clinical outcomes. Their biological behavior is strongly influenced by the tumor microenvironment and intercellular communication, in which extracellular vesicles (EVs) play a crucial role. EV-associated microRNAs (miRNAs) are emerging as potential diagnostic and prognostic biomarkers in glioma patients. In our previous study, we identified CSF-derived miRNA profiles with diagnostic relevance in brain tumors. Here, we extend this work by investigating whether selected miRNAs are also present in EVs circulating in the CSF of glioma patients. Initially, we optimized protocols for EV and miRNA isolation using CSF samples from hydrocephalus patients with sufficient sample volumes. We then analyzed EV-derived miRNAs in a cohort of 101 glioma patients, including 57 with glioblastoma (IDH-wild type), 28 with high-grade gliomas, and 16 with low-grade gliomas. EVs were isolated from 1–4 ml CSF samples, and their purity, size distribution, and concentration were determined. RNA extracted from EVs was used to construct cDNA libraries, which underwent small RNA sequencing after quality control and normalization. Across samples, the most abundant miRNAs included oncogenic species such as miR-21-5p and miR-26a-5p, tumor-suppressive miRNAs including the let-7 family, miR-16, and miR-204, as well as dual-function miRNAs such as miR-125. These findings suggest that glioma cells may utilize EVs to deliver oncogenic miRNAs to recipient cells or to eliminate tumor-suppressive miRNAs, highlighting their diagnostic and mechanistic significance.

Název v anglickém jazyce

Identification of miRNAs with diagnostic potential in extracellular vesicles circulating in cerebrospinal fluid of glioma patients

Popis výsledku anglicky

CzeSEV meeting on Extracellular Vesicles 2025 (ISBN of Proceedings: 978-80-11-07777-8), Vestec, 11.11.2025, poster. Gliomas are highly heterogeneous tumors characterized by diverse molecular and histological profiles, resulting in variable clinical outcomes. Their biological behavior is strongly influenced by the tumor microenvironment and intercellular communication, in which extracellular vesicles (EVs) play a crucial role. EV-associated microRNAs (miRNAs) are emerging as potential diagnostic and prognostic biomarkers in glioma patients. In our previous study, we identified CSF-derived miRNA profiles with diagnostic relevance in brain tumors. Here, we extend this work by investigating whether selected miRNAs are also present in EVs circulating in the CSF of glioma patients. Initially, we optimized protocols for EV and miRNA isolation using CSF samples from hydrocephalus patients with sufficient sample volumes. We then analyzed EV-derived miRNAs in a cohort of 101 glioma patients, including 57 with glioblastoma (IDH-wild type), 28 with high-grade gliomas, and 16 with low-grade gliomas. EVs were isolated from 1–4 ml CSF samples, and their purity, size distribution, and concentration were determined. RNA extracted from EVs was used to construct cDNA libraries, which underwent small RNA sequencing after quality control and normalization. Across samples, the most abundant miRNAs included oncogenic species such as miR-21-5p and miR-26a-5p, tumor-suppressive miRNAs including the let-7 family, miR-16, and miR-204, as well as dual-function miRNAs such as miR-125. These findings suggest that glioma cells may utilize EVs to deliver oncogenic miRNAs to recipient cells or to eliminate tumor-suppressive miRNAs, highlighting their diagnostic and mechanistic significance.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

30204 - Oncology

Projekt

<a href="/cs/project/NU22-03-00290" target="_blank" >NU22-03-00290: Studium exosomálních mikroRNA u gliomů: implikace pro diagnostiku a inovativní terapii</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů