The choice of embedding media affects image quality, tissue R2*, and susceptibility behaviors in post-mortem brain MR microscopy at 7.0T

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064165%3A_____%2F19%3A10393283" target="_blank" >RIV/00064165:_____/19:10393283 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00064190:_____/18:N0000096 RIV/00216208:11110/19:10393283 RIV/00064190:_____/19:N0000066

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=GDRe7_dS8O" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=GDRe7_dS8O</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/mrm.27595" target="_blank" >10.1002/mrm.27595</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The choice of embedding media affects image quality, tissue R2*, and susceptibility behaviors in post-mortem brain MR microscopy at 7.0T

Popis výsledku v původním jazyce

Purpose: The quality and precision of post-mortem MRI microscopy may vary depending on the embedding medium used. To investigate this, our study evaluated the impact of 5 widely used media on: (1) image quality, (2) contrast of high spatial resolution gradient-echo (T-1 and T-2(*)-weighted) MR images, (3) effective transverse relaxation rate (R-2(*)), and (4) quantitative susceptibility measurements (QSM) of post-mortem brain specimens. Methods: Five formaldehyde-fixed brain slices were scanned using 7.0T MRI in: (1) formaldehyde solution (formalin), (2) phosphate-buffered saline (PBS), (3) deuterium oxide (D2O), (4) perfluoropolyether (Galden), and (5) agarose gel. SNR and contrast-to-noise ratii (SNR/CNR) were calculated for cortex/white matter (WM) and basal ganglia/WM regions. In addition, median R-2* and QSM values were extracted from caudate nucleus, putamen, globus pallidus, WM, and cortical regions. Results: PBS, Galden, and agarose returned higher SNR/CNR compared to formalin and D2O. Formalin fixation, and its use as embedding medium for scanning, increased tissue R-2*. Imaging with agarose, D2O, and Galden returned lower R-2* values than PBS (and formalin). No major QSM offsets were observed, although spatial variance was increased (with respect to R-2* behaviors) for formalin and agarose. Conclusions: Embedding media affect gradient-echo image quality, R-2*, and QSM in differing ways. In this study, PBS embedding was identified as the most stable experimental setup, although by a small margin. Agarose and Galden were preferred to formalin or D2O embedding. Formalin significantly increased R-2* causing noisier data and increased QSM variance.

Název v anglickém jazyce

The choice of embedding media affects image quality, tissue R2*, and susceptibility behaviors in post-mortem brain MR microscopy at 7.0T

Popis výsledku anglicky

Purpose: The quality and precision of post-mortem MRI microscopy may vary depending on the embedding medium used. To investigate this, our study evaluated the impact of 5 widely used media on: (1) image quality, (2) contrast of high spatial resolution gradient-echo (T-1 and T-2(*)-weighted) MR images, (3) effective transverse relaxation rate (R-2(*)), and (4) quantitative susceptibility measurements (QSM) of post-mortem brain specimens. Methods: Five formaldehyde-fixed brain slices were scanned using 7.0T MRI in: (1) formaldehyde solution (formalin), (2) phosphate-buffered saline (PBS), (3) deuterium oxide (D2O), (4) perfluoropolyether (Galden), and (5) agarose gel. SNR and contrast-to-noise ratii (SNR/CNR) were calculated for cortex/white matter (WM) and basal ganglia/WM regions. In addition, median R-2* and QSM values were extracted from caudate nucleus, putamen, globus pallidus, WM, and cortical regions. Results: PBS, Galden, and agarose returned higher SNR/CNR compared to formalin and D2O. Formalin fixation, and its use as embedding medium for scanning, increased tissue R-2*. Imaging with agarose, D2O, and Galden returned lower R-2* values than PBS (and formalin). No major QSM offsets were observed, although spatial variance was increased (with respect to R-2* behaviors) for formalin and agarose. Conclusions: Embedding media affect gradient-echo image quality, R-2*, and QSM in differing ways. In this study, PBS embedding was identified as the most stable experimental setup, although by a small margin. Agarose and Galden were preferred to formalin or D2O embedding. Formalin significantly increased R-2* causing noisier data and increased QSM variance.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30224 - Radiology, nuclear medicine and medical imaging

Projekt

<a href="/cs/project/NV15-25602A" target="_blank" >NV15-25602A: Biomarkery progrese a terapeutické odpovědi u neurodegenerativních onemocnění</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Magnetic Resonance in Medicine

ISSN

0740-3194

e-ISSN

—

Svazek periodika

81

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

14

Strana od-do

2688-2701

Kód UT WoS článku

000462092100039

EID výsledku v databázi Scopus

2-s2.0-85057769439