Interleukin-1 Gene Variability and Plasma Levels in Czech Patients with Chronic Periodontitis and Diabetes Mellitus

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064165%3A_____%2F19%3A10396107" target="_blank" >RIV/00064165:_____/19:10396107 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14110/19:00107343 RIV/00159816:_____/19:00071172

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=XxpLSCDDSG" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=XxpLSCDDSG</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1155/2019/6802349" target="_blank" >10.1155/2019/6802349</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Interleukin-1 Gene Variability and Plasma Levels in Czech Patients with Chronic Periodontitis and Diabetes Mellitus

Popis výsledku v původním jazyce

Recent studies have suggested a bidirectional relationship between chronic periodontitis (CP) and diabetes mellitus (DM). Immunoregulatory factors such as cytokines play an important role in etiopathogenesis of both diseases. The aim of this study was to analyze variability in interleukin-1 (IL-1) gene cluster and IL-1 plasma levels in patients with CP, DM, and a combination of both diseases. A total of 1016 individuals participating in this case-control study225 healthy controls, 264 patients with CP, 132 with type 1 diabetes (T1DM), and 395 patients with type 2 diabetes (T2DM)were genotyped using methods based on polymerase chain reaction for IL-1 gene polymorphisms (IL-1A (-889C/T, rs1800587), IL-1B (+3953C/T, rs1143634), and IL-1RN (gene for IL-1 receptor antagonist, IL-1RA, 86bp tandem repeats in intron 2)). Levels of IL-1 were measured by Luminex methods in subgroups of controls, CP, T1DM+CP, and T2DM+CP subjects. Although no significant associations were found in the genotype and allele frequencies of IL-1A (-889C/T), significant differences in the allele frequencies of IL-1B (+3953C/T) were observed between controls and CP patients (P&lt;0.05). In T1DM patients, IL-1RNS short allele and IL-1RN 12 genotype were significantly less frequent than those in controls (P&lt;0.01). In haplotype analysis, TTL haplotype decreased the risk of CP development (P&lt;0.01), whereas CCS and CTL haplotypes (P&lt;0.01 and P&lt;0.05) were associated with T1DM. Although IL-1 levels were measured significantly higher in mononuclear cells after stimulation by mitogens, HSP70, or selected periodontal bacteria than in unstimulated cells, IL-1 genotypes did not correlate with circulating IL-1 levels. In the Czech population, significant associations between the IL-1B polymorphism with CP and the IL-1RN variant with T1DM were found. Haplotype analysis suggests that variability in IL-1 gene cluster may be one of the factors in the CP and T1DM pathogenesis, although single variants of these polymorphisms are not substantial for protein production.

Název v anglickém jazyce

Interleukin-1 Gene Variability and Plasma Levels in Czech Patients with Chronic Periodontitis and Diabetes Mellitus

Popis výsledku anglicky

Recent studies have suggested a bidirectional relationship between chronic periodontitis (CP) and diabetes mellitus (DM). Immunoregulatory factors such as cytokines play an important role in etiopathogenesis of both diseases. The aim of this study was to analyze variability in interleukin-1 (IL-1) gene cluster and IL-1 plasma levels in patients with CP, DM, and a combination of both diseases. A total of 1016 individuals participating in this case-control study225 healthy controls, 264 patients with CP, 132 with type 1 diabetes (T1DM), and 395 patients with type 2 diabetes (T2DM)were genotyped using methods based on polymerase chain reaction for IL-1 gene polymorphisms (IL-1A (-889C/T, rs1800587), IL-1B (+3953C/T, rs1143634), and IL-1RN (gene for IL-1 receptor antagonist, IL-1RA, 86bp tandem repeats in intron 2)). Levels of IL-1 were measured by Luminex methods in subgroups of controls, CP, T1DM+CP, and T2DM+CP subjects. Although no significant associations were found in the genotype and allele frequencies of IL-1A (-889C/T), significant differences in the allele frequencies of IL-1B (+3953C/T) were observed between controls and CP patients (P&lt;0.05). In T1DM patients, IL-1RNS short allele and IL-1RN 12 genotype were significantly less frequent than those in controls (P&lt;0.01). In haplotype analysis, TTL haplotype decreased the risk of CP development (P&lt;0.01), whereas CCS and CTL haplotypes (P&lt;0.01 and P&lt;0.05) were associated with T1DM. Although IL-1 levels were measured significantly higher in mononuclear cells after stimulation by mitogens, HSP70, or selected periodontal bacteria than in unstimulated cells, IL-1 genotypes did not correlate with circulating IL-1 levels. In the Czech population, significant associations between the IL-1B polymorphism with CP and the IL-1RN variant with T1DM were found. Haplotype analysis suggests that variability in IL-1 gene cluster may be one of the factors in the CP and T1DM pathogenesis, although single variants of these polymorphisms are not substantial for protein production.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30208 - Dentistry, oral surgery and medicine

Projekt

<a href="/cs/project/GB14-37368G" target="_blank" >GB14-37368G: Centrum orofaciálního vývoje a regenerace</a><br>

Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Dentistry

ISSN

1687-8728

e-ISSN

—

Svazek periodika

2019

Číslo periodika v rámci svazku

January

Stát vydavatele periodika

EG - Egyptská arabská republika

Počet stran výsledku

10

Strana od-do

6802349

Kód UT WoS článku

000457007900001

EID výsledku v databázi Scopus

2-s2.0-85060815678